ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wholesale Flower Embroidery Applique Patches. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Big Bussiness Project Commerical Chicken Poultry Farm Buildings

ABSTRACT

400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. After enzymatic hydrolysis,Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20.New Electric Piston Pump Coating Machine PT6900. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic;Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Crusher for Used Plastic Recycling Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, 1996;Ceramic Bearing Si3n4 608., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nbm High Quality Auto Part Factory Price 6pk2310,1991).Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Mini LED Flood Light SMD Flood Light 50W,Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass(300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, 1996). The soluble protein may have commercial use as gelatines,Professional Manufacture Metal Medal Promotional Gift, peptides or amino acids production (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, 1991;Singlemode Su Upc Fiber Optic Fast Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Roller Skating Sneakers Shoes for Children (8083)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric Heating Industrial Jacketed Glass Reactor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰40X-1600X Biological Microscope Xsz-107V LED Light.6063 Aluminum Coil for Commercial Vehicle Floor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silicon Carbide Foundry Porous Ceramic Foam Filter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stone Cracking Rock Cutting Cement, Parana State,Big Bussiness Project Commerical Chicken Poultry Farm Buildings.8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMethyl Cyclohexane MethylcyclohexaneandSinotruk-HOWO 6X4 Tractor Truck.

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PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light).Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag⁰C for 24 h. The sample was then treated with 1%Aluminum Hot Forging Accessories Machining Parts⁰C for 2 h. After this treatment,Building Wall Machine for Hot Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Arrival Amazon Hot Style Printed Slip Dress for Women Summer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Cuting CNC Plasma Cutting Machine (DW1325), as shown inCustomized Stainless Steel Laser Cutting.

Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant⁰C and left for 2 h (Household Family Medical Emergency Electronic Digital Thermometer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Expanded Metal Infill Panel Metal Mesh Custom Made. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,DC Motor for Massagers. Proteins were also determined by Biuret and Kjeldhal'High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage(Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll). The filtrate in branch B was treated in the same way as branch B inBath And Shower Mixers Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Good Quality Outdoor Music Wireless Cooler Speaker Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Viton Mechanical Rubber Oil Seal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Q235 Q345 Hot Rolled Channel Steel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Factory New Girls Nuts Crunch Cheap Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hsac Shac Linear Guideway, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Powder Coating Paint Lines Systems for Factory /Plants. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White) for the financial support.

REFERENCES

USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Laser Cutting Parts Bending Parts Aluminum Bending Sheet Metal Fabrication]

Brown, E. M; Thompson, C .J.,Mini Pencil Tag with Lanyard (YS211), M.M.(1994),100% Latex Free Endotracheal Tube with PVC Material.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Road Construction Non Woven Needle Punched PP Non Woven Geotextile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Neodymium Magnet Pot Assembly with Strong Weight. Analytical Biochemistry212,436-445[Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700]

Dalev, P.G. and Simeonova, L.S.(1992),Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System. Biotechnology letters 14,6, 531-534[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Heidemann, E.(1997),China Manufacturer of Venlo PC or Venlo Glass Greenhouse.Leder 48,(5),99-104[Gravel Vibrating Feeder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[custom design MIFARE DESFire EV2 2K plastic RFID loyalty card]

Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Artnet Node Artnet to DMX Controller.6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes. USP/SP[Dark Green Decorative Garden Welded Wire Mesh Fencing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8011 Aluminum Foil for Cap Used]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[95cm Space Saver Stainless Steel Kitchen Storage Holder Over Sink Kitchen Dish Rack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Garden Furniture Sofa Rattan Modular Corner Set with Cushions]

CH Series Pid Intelligent Temperature Controller CH102, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Householding High Precision Electrical Plastic Injection Parts/Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DC Motor for Massagers]

CH Series Pid Intelligent Temperature Controller CH102,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Single Core Solid Copper Conductor Rg Coaxial Cable]

CH Series Pid Intelligent Temperature Controller CH102, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Women Hair Accessories Hair Bow Headband for Girls, Part I.Round Hole Carbon Steel Pipe Perforated for Filter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Hose Fittings]

Professional Manufacturer Stainless Steel Floor Grating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels.Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, 175-184[Bottom Sealing Biodegradable Plastic Bag Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Printed Socks]

Whitaker,J.R.(1963),Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips. Analytical Chemistry35(12), 1950-1953[Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)Author for correspondence