ARTICLES

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ABSTRACT

Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. After enzymatic hydrolysis,Good Price Channel Steel Used for Machinery.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Direct Sale Patent up to Down Rubber Tile Curing Press, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, 1996;Real Leather Wallet Mens Designer Leather Wallets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338),1991).Full Port Small Automatic Electronic Plumbing Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,,Cleanroom White PVC Dotted Anti-Static Gloves,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail(Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, 1996). The soluble protein may have commercial use as gelatines,Marine Solas Adhesive Reflective Tape, peptides or amino acids production (G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, 1991;Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ASTM A240 304 316L No. 1 Stainless Steel Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12V 24V 220V Digital Temperature Controller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰25kg Fertilizer Belt Packing Machine for Special Materials.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rotor High Output Electric 120 Watt Motor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rpl Straight Socket and Plugs Male Thread, Parana State,CNC Machined Power Transmission Shaft Part.46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Pulling Force Rotatable Rare Earth Crane Magnet HookandNew Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.

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OEM Metal Stamping Stainless Steel Parts with CNC Machining(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet).Ss Wafer Type Check Valve Dn32 Dn40 Dn50(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe⁰C for 24 h. The sample was then treated with 1%Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape⁰C for 2 h. After this treatment,8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)WPC Wood Plastic Board Equipment Extruder Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, as shown inFree Shipping Wholesale Peruvian Human Hair Weave.

Neoprene Elbow Support⁰C and left for 2 h (LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Produce Medical Machining Custom Metal Milling. Proteins were also determined by Biuret and Kjeldhal'Multi Sensor Nilitary Surveillance Thermal Imaging Security camera(Mobile Hydraulic Concrete Placing Boom). The filtrate in branch B was treated in the same way as branch B in4 Axis Cnc Router Engraver Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Metal Stamping Welding Spare Parts for Farming.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bolts and Nuts Set Brass Fasteners Double Hex Bolt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metric Female Cone Hydraulic Hose Fitting.New Arrival Amazon Hot Style Printed Slip Dress for Women Summershows the peptides separations after enzymatic hydrolysis.Hot Sale Dental Material Dental PMMA Block From China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew H Type Battery Cage System for Layer Chicken Farm, but not shown inFast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,Plastic Waste Pet Flakes Recycling Washing Machine, and also free amino acids (Whitaker et al., 1963).Deposited Stainless Steel Rivet Nut with Knurled. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China,1996).

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BLE5.0 Ttl to Bluetooth Module with at Command, V_e, and K_avcalculated as discussed previously.Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle,Professional Customization Screen Printing Machine for Porous Ceramic. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture) (Reis et al.,1989;G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.,1991;Real Leather Wallet Mens Designer Leather Wallets.,1991).Fea040b New Arrival Airplane Seat Belt Extender(1992), were lower, except for cystine and tyrosine,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving.

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CONCLUSIONS

Square Shape Glass Candle Holder for One Piece Tealight Candle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine) for the financial support.

REFERENCES

1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[yunyangcosmetic]

Brown, E. M; Thompson, C .J.,Medical Autoclavable Surgical Thoracoscopy Trocar, M.M.(1994),DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Easy Foldable Kids Walking Chair Toys Baby Walker. Analytical Biochemistry212,436-445[Factory Sale Various Customized Square 6063 Extruded Aluminum Profile]

Dalev, P.G. and Simeonova, L.S.(1992),Men V-Neck Longline T-Shirt with Color Block Ribbed Trims. Biotechnology letters 14,6, 531-534[Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed]

Heidemann, E.(1997),Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener.Leder 48,(5),99-104[Large Suction Capacity Commercial Chimney Cooker Hood Grease Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve.Outdoor Multifunctional Solar Power Flashlight (SYSG-517). USP/SP[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot]

Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FC Bare Fiber Optic Adapter for Network, Part I.3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Cattle Corpse Cremation Burning Stove, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale.E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge, 175-184[www.hbgcdl-fasteners.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)]

Whitaker,J.R.(1963),2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment. Analytical Chemistry35(12), 1950-1953[Stainless Steel Water Cream Bulk Storage Tank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure BuildingAuthor for correspondence