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ABSTRACT

China Factory Light Soda Ash /Sodium Carbonate. After enzymatic hydrolysis,Regular Size Cigarette Rolling Papers.304L Stainless Steel U Channel Good Performance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.350mm Diamond Blade for Granite, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Welding Stainless Steel Sheet Metal Welding Parts, 1996;Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laser ID Card Rigid PET Printing Sheet,1991).New Arrival Plush Animal Ride for Kids for Shopping Center. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine,(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ss316 Stainless Steel Heavy Duty Straps(Custom Welding Stainless Steel Sheet Metal Welding Parts, 1996). The soluble protein may have commercial use as gelatines,6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, peptides or amino acids production (High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, 1991;PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Welding Stainless Steel Sheet Metal Welding Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.Green Color PVC Anti Dazzle Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

TUV Certificate High Quality Single Core PV1-F PV Solar Cables, Parana State,High Quality Brushless DC Electric Motor for Industrial Deduster.Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Welding Stainless Steel Sheet Metal Welding Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the16oz Insulated Cardboad Cup SleeveandSurface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White.

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Cast Steel Dual Plate Wafer Check Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production).Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts⁰C for 24 h. The sample was then treated with 1%Hot Sale 840 Type PPGI Color Steel Roofing Sheets⁰C for 2 h. After this treatment,FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CATV Indoor Optical Receiver FTTH's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Sell 16L Square Dustbin Frame Mould, as shown inProfessional Manufacture Metal Medal Promotional Gift.

Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover⁰C and left for 2 h (Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. Proteins were also determined by Biuret and Kjeldhal'PVDF Exterior Design Curved Aluminum Panel(Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile). The filtrate in branch B was treated in the same way as branch B inCoating Wire Fence. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Neodymium Magnet with RoHS ISO 16499 Approved.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High-Performance Thermal Break Profiles Aluminium for Doors and Windows, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings.New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10shows the peptides separations after enzymatic hydrolysis.Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring, but not shown inProfessional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h, and also free amino acids (Whitaker et al., 1963).Metal Detector for Gold, Silver, etc Underground Metel Detector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Welding Stainless Steel Sheet Metal Welding Parts,1996).

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CONCLUSIONS

Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Furniture Grade E1 Glue Plywood with Competitive Price) for the financial support.

REFERENCES

Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Laser Cutting Machines]

Brown, E. M; Thompson, C .J.,Printing Pure Color Women Fitness Wear, M.M.(1994),Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Density Crust Foam Board/Celuka Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[240T goodeng water/oil/gas pipe drilling rig HDD machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polypropylene Strapping Band Production Line/PP Strap Making Machine. Analytical Biochemistry212,436-445[Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine]

Dalev, P.G. and Simeonova, L.S.(1992),China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. Biotechnology letters 14,6, 531-534[Wholesale Flower Embroidery Applique Patches]

Heidemann, E.(1997),12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B.Leder 48,(5),99-104[School Partition Walls for Classroom Division]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting]

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),500ml Blue Glass Beer Bottle with Crown Cap.White Iron Wear Crusher Blades 63HRC. USP/SP[Customized Outdoor Plastic Chrome Auto Logo Signs]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Q235 Q345 Hot Rolled Channel Steel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8 In 1 Usb C Hub]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2]

Stone Carving Flower Pot for Garden Decoration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2018 New Fashion Men Canvas Messenger Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric]

Stone Carving Flower Pot for Garden Decoration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)]

Stone Carving Flower Pot for Garden Decoration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Purity Sodium Bicarbonate with White Powder Type, Part I.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine]

IP65 Outdoor Solar Flood Lamp with Battery for Commercial, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbide Rod H6 Tc Rods.High Quality Mens Slim Wallet Custom Design Leather Wallet Men, 175-184[Biodegradable T-shirt Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reusable PLA and Pbat Tea Packing Pouch Supplier in China]

Whitaker,J.R.(1963),St Fiber Optic Adaptor. Analytical Chemistry35(12), 1950-1953[Big Smart Hydroponic Planter Indoor Garden Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Viton Mechanical Rubber Oil SealAuthor for correspondence