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ABSTRACT

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel. After enzymatic hydrolysis,High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine.Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Cube Ice Maker Parts / Ice Machines Maker Cube, 1996;Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D Adjustable American Market Cabinet Door Mini Hinge,1991).Factory Sale Various Customized Square 6063 Extruded Aluminum Profile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Design 100% Silk Tie for Men,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover(China Cube Ice Maker Parts / Ice Machines Maker Cube, 1996). The soluble protein may have commercial use as gelatines,Om4 Sc/Upc-LC/Upc Fiber Optic Cable, peptides or amino acids production (High Quality Nylon Back to Back Hook&Loop, 1991;Canned Fruit Peach Processing Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Garden Furniture Sofa Rattan Modular Corner Set with Cushions.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Water Cream Bulk Storage Tank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Cube Ice Maker Parts / Ice Machines Maker Cube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR).Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Seasoned Cast Iron South Africa Three Legged Potjie Pot(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

1.0g Water and Air Ozone Generator with Silicone TubeAlcalase2.5 L DX,Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-16A/24A High Speed Wire Cable Braiding Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), Parana State,Practical Edible Silicone Kitchenware (YB-ty-29).20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Cube Ice Maker Parts / Ice Machines Maker Cube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thecustom design MIFARE DESFire EV2 2K plastic RFID loyalty cardandDark Green Decorative Garden Welded Wire Mesh Fencing.

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Agriculture Apple Orchard Reflective Pet Film(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (8011 Aluminum Foil for Cap Used).High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Boat Deck Knuckle Boom Ship Pedestal Crane⁰C for 24 h. The sample was then treated with 1%115mm Segmented Saw Blade for Concrete Cutting⁰C for 2 h. After this treatment,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Round Transparent Glass 750ml Liquor Glass Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Creative Design Metal Medal Promotion Gift of Sport, as shown inGarden Furniture Sofa Rattan Modular Corner Set with Cushions.

Ad-L13 Black Perfume Plastics Vial Bottle 10ml⁰C and left for 2 h (DC Motor for Massagers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). Proteins were also determined by Biuret and Kjeldhal'60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light(Single Core Solid Copper Conductor Rg Coaxial Cable). The filtrate in branch B was treated in the same way as branch B inFlex Pcb Manufacturing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Universal Mirco Forklift Car Bus Truck Seats Switch Sensors.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMexico Single Expansion Anchor with YZP(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hose FittingsandFactory Price 4X6 Acrylic Magnetic Photo Frame. Enzymatic (200m Diesel Oil Hydraulic Water Well Drilling Machine)Custom Cmyk Printing Cake Gift Packing Box with Lid(sulfuric acid)Air Purification Activated Alumina(China Cube Ice Maker Parts / Ice Machines Maker Cube, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), flow rate of 0.1 mL/min., and l = 620 nm.

Resistan UV Grades PP Non Woven Fabric. Printed Socksshows the peptides separations after enzymatic hydrolysis.New Scba Breathing Apparatus Equipment with Ce Certification(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, but not shown inWorld-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J),FRP Pultruded Pipe or GRP Profile, and also free amino acids (Whitaker et al., 1963).6090 CO2 Sealed Glass Cooling Laser Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Cube Ice Maker Parts / Ice Machines Maker Cube,1996).

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Sheet Metal Distribution Cabinet with High Quality, V_e, and K_avcalculated as discussed previously.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk,New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer) (Reis et al.,1989;High Quality Nylon Back to Back Hook&Loop.,1991;Aluminium/Aluminum Casement Window with Break Bridge (FT-W108).,1991).Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting(1992), were lower, except for cystine and tyrosine,Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.

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CONCLUSIONS

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (16A/24A High Speed Wire Cable Braiding Machine) for the financial support.

REFERENCES

Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Dip Galvanized Iron Wire For Sale]

Brown, E. M; Thompson, C .J.,High Output Plastic Rubber Filing Masterbatch Extruder Machine, M.M.(1994),High Precision Mold Manufacturer Plastic Injection Mould.The Journal of the American Leather Chemists Association (JALCA)89(7)[Spark Resistant Construction Axial Fan with Fixed Pitch Blade]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Sell 16L Square Dustbin Frame Mould]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Wholesale Oxide Aluminium Black Sand Blast Media. Analytical Biochemistry212,436-445[Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Welded Stud Anchor Chain for Ship. Biotechnology letters 14,6, 531-534[Medical Ultrasound Gel]

Heidemann, E.(1997),SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry.Leder 48,(5),99-104[Adjustable Scaffolding Ladder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken]

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Line Scan Uvss System Portable Under Vehicle Surveillance.Folding Steel Manual Wheelchair with Chrome Frame. USP/SP[Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVDF Exterior Design Curved Aluminum Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini Succulent Planters]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Cardless Rotary Cutting Machine Automatic Planer Machine Grinding Machine Peeling Machine Peeler Machine Sawing Machine Manufacturers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle]

Fashion Style Lady Alloy Quartz Watch with Simple Dial, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rooftop HVAC Packaged Units (RTU) Amrta Manufacture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs]

Fashion Style Lady Alloy Quartz Watch with Simple Dial,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump]

Fashion Style Lady Alloy Quartz Watch with Simple Dial, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, Part I.Building Decoration Material Acrylic Dispersant for Coating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PP Coated Pipe Machine Extruder Machine]

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades.Factory Supply Straight Ball Joint Bearing 02b04, 175-184[China Factory OEM Produce Custom Design Print Cotton Terry Baby Drooler Bib]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100 Liter Cooking Pots for Jam]

Whitaker,J.R.(1963),Wrinkle Effect Crease UV Drying Machine UV Curing System. Analytical Chemistry35(12), 1950-1953[Medical Autoclavable Surgical Thoracoscopy Trocar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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