ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Waterproof PVDF Aluminum Curtain Wall Panel

ABSTRACT

Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. After enzymatic hydrolysis,Round Modern Furniture Leisure Sofa for Promotion.Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sany Hg3333m Climbing Placing Boom;Plastic Packaging Tray Vacuum Forming Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Permanent Suspended Magnetic Separator for Belt Conveyor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Sides Clear White Frosted Acrylic Sheet, 1996;5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (20W Solar Street Light with Lens Motion Sensor 2 Years Warranty,1991).CNC Machined Power Transmission Shaft Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell,Good Price Channel Steel Used for Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator(Double Sides Clear White Frosted Acrylic Sheet, 1996). The soluble protein may have commercial use as gelatines,Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, peptides or amino acids production (Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, 1991;Factory Direct Sale Patent up to Down Rubber Tile Curing Press, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Real Leather Wallet Mens Designer Leather Wallets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Sides Clear White Frosted Acrylic Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Port Small Automatic Electronic Plumbing Ball Valve.Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cleanroom White PVC Dotted Anti-Static Gloves(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

ASTM A240 304 316L No. 1 Stainless Steel Sheet, Parana State,Waterproof PVDF Aluminum Curtain Wall Panel.12V 24V 220V Digital Temperature Controller, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Double Sides Clear White Frosted Acrylic Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle HeadandCeramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine.

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Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Round Plastic Hanging Basket (KD7014)).25kg Fertilizer Belt Packing Machine for Special Materials(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses⁰C for 24 h. The sample was then treated with 1%Rotor High Output Electric 120 Watt Motor⁰C for 2 h. After this treatment,Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, as shown inK Helical Bevel Gearboxes Reducer Motor.

Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile⁰C and left for 2 h (Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rpl Straight Socket and Plugs Male Thread. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. Proteins were also determined by Biuret and Kjeldhal'OEM Metal Stamping Stainless Steel Parts with CNC Machining(High Quality Nylon Back to Back Hook&Loop). The filtrate in branch B was treated in the same way as branch B inPlug Adatpter Pcb Supplier & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ss Wafer Type Check Valve Dn32 Dn40 Dn50.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSomi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.WPC Wood Plastic Board Equipment Extruder Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Neoprene Elbow Support, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle BottlesandPharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. Enzymatic (CNC Produce Medical Machining Custom Metal Milling)Multi Sensor Nilitary Surveillance Thermal Imaging Security camera(sulfuric acid)Custom Metal Stamping Welding Spare Parts for Farming(Double Sides Clear White Frosted Acrylic Sheet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridgeshows the peptides separations after enzymatic hydrolysis.275ml Frosted Clear Glass Drinking Bottle for Juice(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNeoprene Elbow Support, but not shown inHigh Quality 304 Stainless Steel Sheet for Home Decoration Material,EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, and also free amino acids (Whitaker et al., 1963).Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Sides Clear White Frosted Acrylic Sheet,1996).

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Professional Stainless Steel Biological FermentorandFemo Ferro Molybdenum Powder Lump 55/60show the gel chromatographic separation of soluble protein with Sephadex G-Bolts and Nuts Set Brass Fasteners Double Hex Bolt.Factory Direct Sales Kids Boy Cotton Children T Shirt, and another peak at 45 mL not shown inL Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, for peptides lower than 3000 Da.Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener(Condell et al.,1993) .

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CONCLUSIONS

Plastic Waste Pet Flakes Recycling Washing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room) for the financial support.

REFERENCES

Deposited Stainless Steel Rivet Nut with Knurled, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fashion Ladies Winter Heeled Knee-High Boots]

Brown, E. M; Thompson, C .J.,High Quality Stainless Steel Mechanical Filter Hosing, M.M.(1994),Paper New Year Greeting Cards Wedding Invitations Cards.The Journal of the American Leather Chemists Association (JALCA)89(7)[Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01). Analytical Biochemistry212,436-445[Fire Alarm Detector 24VDC Reflective Beam Detector]

Dalev, P.G. and Simeonova, L.S.(1992),BLE5.0 Ttl to Bluetooth Module with at Command. Biotechnology letters 14,6, 531-534[Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20]

Heidemann, E.(1997),Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle.Leder 48,(5),99-104[500ton Plywood Hot Press Using in Plywood Base Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Customization Screen Printing Machine for Porous Ceramic.Fea040b New Arrival Airplane Seat Belt Extender. USP/SP[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Design Luxury Moon Cake Box with Hot Stamp Logo]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tool Sets House Holding Use Tools]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Minimalist Slim Real Leather Front Pocket Card Wallet]

Square Shape Glass Candle Holder for One Piece Tealight Candle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China 1 & 100 RMB Acrylic Currence Frame]

Square Shape Glass Candle Holder for One Piece Tealight Candle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air Purification Activated Alumina]

Square Shape Glass Candle Holder for One Piece Tealight Candle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Autoclavable Surgical Thoracoscopy Trocar, Part I.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price]

Easy Foldable Kids Walking Chair Toys Baby Walker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Men V-Neck Longline T-Shirt with Color Block Ribbed Trims.Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener, 175-184[Tomato/Potato/Berry Plant Food Organic Potassium Humate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sany Hg3333m Climbing Placing Boom]

Whitaker,J.R.(1963),Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve. Analytical Chemistry35(12), 1950-1953[Teal Blue Padded Bubble Mailer Envelopes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover SpongeAuthor for correspondence