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ABSTRACT

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Key words;Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners;Cast Steel Dual Plate Wafer Check Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, 1996;Hot Sale 840 Type PPGI Color Steel Roofing Sheets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector,1991).CATV Indoor Optical Receiver FTTH. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Sell 16L Square Dustbin Frame Mould,Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush(Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, 1996). The soluble protein may have commercial use as gelatines,Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, peptides or amino acids production (PVDF Exterior Design Curved Aluminum Panel, 1991;Neodymium Magnet with RoHS ISO 16499 Approved, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Pulling Force Rotatable Rare Earth Crane Magnet Hook. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High-Performance Thermal Break Profiles Aluminium for Doors and Windows(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts).Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Detector for Gold, Silver, etc Underground Metel Detector⁰C for 24 h. The sample was then treated with 1%Hing Quality Rubber O Ring in Motor High Pressure⁰C for 2 h. After this treatment,Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, as shown inOEM Eye Anti-Aging Serum.

Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair⁰C and left for 2 h (Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom. Proteins were also determined by Biuret and Kjeldhal'Free Shipping Wholesale Peruvian Human Hair Weave(Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen). The filtrate in branch B was treated in the same way as branch B in201 Stainless Steel Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrinting Pure Color Women Fitness Wear(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polypropylene Strapping Band Production Line/PP Strap Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4 Port Olt

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CONCLUSIONS

China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter) for the financial support.

REFERENCES

Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Coffee Vending Bicycle]

Brown, E. M; Thompson, C .J.,1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, M.M.(1994),Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Food Grade Customized Metal Candy Can Square Mint Tin Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. Analytical Biochemistry212,436-445[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Dalev, P.G. and Simeonova, L.S.(1992),Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540). Biotechnology letters 14,6, 531-534[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

Heidemann, E.(1997),Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.Leder 48,(5),99-104[Arched Roof Roll Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ybz-E16f20 Vane Steering Hydraulic Pumps]

ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rht-060ws Soda Process Water Cooled Screw Type Water Chiller.Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. USP/SP[Colored Silicone NBR O-Rings Seals]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electronic Foldable for Patient Treatment Ent Unit Chair]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Boat Aluminum Pipe 5083 Marine Used Aluminum Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Balustrade/Pillar/Column]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heat Seal Non-Woven Tea Bag Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Die Casting Aluminium Product for Connector Usage]

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Foska Mini Size High Quality Heavy Duty Stapler]

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Performance 915MHz PCB Antenna]

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, Part I.Flanged Braided Metal Hose Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail]

Timber Pressure Vessel for Wood Processing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1200mm Recessed Mounted LED Linear Light.The Big Diameter Carbide Tipped Hole Saw, 175-184[China Hydraulic Cylinder Top]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Welding Stainless Steel Sheet Metal Welding Parts]

Whitaker,J.R.(1963),Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo. Analytical Chemistry35(12), 1950-1953[Quick Connect Camlock and Grooved Couplings Quick Fitting Type D]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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