ARTICLES

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ABSTRACT

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. After enzymatic hydrolysis,Marine Solas Adhesive Reflective Tape.G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12V 24V 220V Digital Temperature Controller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, 1996;25kg Fertilizer Belt Packing Machine for Special Materials., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses,1991).Rotor High Output Electric 120 Watt Motor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby,Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine(Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, 1996). The soluble protein may have commercial use as gelatines,Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, peptides or amino acids production (Rpl Straight Socket and Plugs Male Thread, 1991;46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Metal Stamping Stainless Steel Parts with CNC Machining., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ss Wafer Type Check Valve Dn32 Dn40 Dn50.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:WPC Wood Plastic Board Equipment Extruder Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, Parana State,Cleanroom White PVC Dotted Anti-Static Gloves.Custom Metal Stamping Welding Spare Parts for Farming, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Band Cable Ties Wire Strap Steel BandingandFull Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse.

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Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Permanent Suspended Magnetic Separator for Belt Conveyor).Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%275ml Frosted Clear Glass Drinking Bottle for Juice⁰C for 24 h. The sample was then treated with 1%EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price⁰C for 2 h. After this treatment,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bolts and Nuts Set Brass Fasteners Double Hex Bolt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Direct Sales Kids Boy Cotton Children T Shirt, as shown inCarbon Steel Stainless Steel Countersunk Head Hollow Bolt.

Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener⁰C and left for 2 h (Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Light Blue PVC Plastic Sheet and PVC Film. Proteins were also determined by Biuret and Kjeldhal'Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy(Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)). The filtrate in branch B was treated in the same way as branch B inSnack Foods and Sauces. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Metric Female Cone Hydraulic Hose Fitting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Dental Material Dental PMMA Block From China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Waste Pet Flakes Recycling Washing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Stainless Steel Mechanical Filter Hosing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Paper New Year Greeting Cards Wedding Invitations Cards, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Square Shape Glass Candle Holder for One Piece Tealight Candle.1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tankshows the peptides separations after enzymatic hydrolysis.1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCATV Indoor Optical Receiver FTTH, but not shown inA4 Diploma Covers Wholesale with Silver Foil,Medical Autoclavable Surgical Thoracoscopy Trocar, and also free amino acids (Whitaker et al., 1963).DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price,1996).

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CONCLUSIONS

FC Bare Fiber Optic Adapter for Network. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules) for the financial support.

REFERENCES

3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.nsen-valve.com]

Brown, E. M; Thompson, C .J.,Cattle Corpse Cremation Burning Stove, M.M.(1994),L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Longyi Crimping Types Cable Terminal Lugs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge. Analytical Biochemistry212,436-445[Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment. Biotechnology letters 14,6, 531-534[Die Cast Iron Worm Gear Speed Reduce Gearbox]

Heidemann, E.(1997),Professional Stainless Steel Biological Fermentor.Leder 48,(5),99-104[Bluestone Antique Finishing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Flange Dismantling Joint]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[South Africa power cord]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors]

16 Spindle Lace High Speed Braiding Machine Textile Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Micro Button Type Load Cell Compression Load Cell 10kg-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124]

16 Spindle Lace High Speed Braiding Machine Textile Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ring Caps &Plastic Tube Pop Novelties]

16 Spindle Lace High Speed Braiding Machine Textile Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building, Part I.Interlocking Precast Plastic Concrete Paver Molds for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ent Treatment Medical Chair with Foot Control]

24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna.Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe, 175-184[Pcba Clone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Noiseless) Cracking Agent (SCA) for Breaking Rocks]

Whitaker,J.R.(1963),Plastic Flange Housing Ucf310-115 for Electric Hand Tools. Analytical Chemistry35(12), 1950-1953[Factory Direct Sales Kids Boy Cotton Children T Shirt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High-Performance Thermal Break Profiles Aluminium for Doors and WindowsAuthor for correspondence