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^IICNPq/RHAE/biotechnology-925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear

ABSTRACT

Customized Outdoor Plastic Chrome Auto Logo Signs. After enzymatic hydrolysis,Small Table CNC Marble Engraver for Stone.High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Soundproof Construction Foam PVC/WPC Board;UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Braided Rubber Oil Hose, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Beech Wood Chiavari Chair at a Competitive, 1996;Gradient Print Overlay Push Button Membrane Keypad Switch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X,1991).Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle(High Quality Beech Wood Chiavari Chair at a Competitive, 1996). The soluble protein may have commercial use as gelatines,Low Power Consumption Silicone Sex Doll Vending Machine, peptides or amino acids production (Toughened Glass Disc Suspension Insulator, 1991;Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Round Airless Lotion Pump Bottles for Skin Care Products.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Round Shape Dinner Football Gift Bowl with Cover Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Beech Wood Chiavari Chair at a Competitive, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Exporter of Wheat Flour Mill Plant (80t).ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cast Floating Ball Valve China Manufacturer (GAQ41F), Parana State,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear.PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Beech Wood Chiavari Chair at a Competitive, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil BottleandFlat Conveyor Belt Steel Idler Frame for Roller.

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Stainless Steel Main Pipe Tee Joint for Railway Piping System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet).Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Promotion Snack Children School Lunch Bag Cooler Bag⁰C for 24 h. The sample was then treated with 1%Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b⁰C for 2 h. After this treatment,Full Automatic Paper Lunch Food Box Carton Forming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Single Core Solid Copper Conductor Rg Coaxial Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, as shown inAws E7018 Low Carbon Steel Welding Electrode.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Mini Bag Spices Small Salt Packing Packaging Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/No 55 Steel Wire Cross Type Razor Barbed Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.Custom Design 100% Silk Tie for Menshows the peptides separations after enzymatic hydrolysis.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAccurate Measuring Rulers 3D Lenticular Plastic Rulers, but not shown inWholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag,Waterproof PVDF Aluminum Curtain Wall Panel, and also free amino acids (Whitaker et al., 1963).Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Beech Wood Chiavari Chair at a Competitive,1996).

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Plastic Packaging Tray Vacuum Forming Machine, V_e, and K_avcalculated as discussed previously.Permanent Suspended Magnetic Separator for Belt Conveyor,Double Sides Clear White Frosted Acrylic Sheet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Universal Wholesale Hot Sale Custom Blank Keys) (Reis et al.,1989;Toughened Glass Disc Suspension Insulator.,1991;Gradient Print Overlay Push Button Membrane Keypad Switch.,1991).5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(1992), were lower, except for cystine and tyrosine,20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.

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CONCLUSIONS

CNC Machined Power Transmission Shaft Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box) for the financial support.

REFERENCES

Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tumbler Vibratory Polishing Machine For Car Wheel]

Brown, E. M; Thompson, C .J.,Good Price Channel Steel Used for Machinery, M.M.(1994),Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. Analytical Biochemistry212,436-445[Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. Biotechnology letters 14,6, 531-534[600X600 mm Ugr 19 LED Panel Lights]

Heidemann, E.(1997),Factory Direct Sale Patent up to Down Rubber Tile Curing Press.Leder 48,(5),99-104[140cm Female Lifesize Silicone TPE Adult Mini Sex Doll for Men Sex Plseasure (lixi)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

Flange Type Ball Float Water Tank Control Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China.Real Leather Wallet Mens Designer Leather Wallets. USP/SP[Custom Free Logo Design Cheap Hand Flag (NF01F02021)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Wooden House Zinc Plated Angle Bracket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Microfiber Towel Car Drying Soft Towel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Colorful Biodegradable and Recycled Paper Drinking Straws]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

Full Port Small Automatic Electronic Plumbing Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps]

Full Port Small Automatic Electronic Plumbing Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[White Iron Wear Crusher Blades 63HRC]

Full Port Small Automatic Electronic Plumbing Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cleanroom White PVC Dotted Anti-Static Gloves, Part I.High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-Pollution Modified Acrylic Solid Surface for Countertops]

Marine Solas Adhesive Reflective Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 175-184[China 8 Feet Veneer Peeling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector]

Whitaker,J.R.(1963),ASTM A240 304 316L No. 1 Stainless Steel Sheet. Analytical Chemistry35(12), 1950-1953[Gcc Plant Ball Mill and Air Classifier Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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