ARTICLES

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ABSTRACT

PP Coated Pipe Machine Extruder Machine. After enzymatic hydrolysis,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts.Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mining Machinery Parts Diamond Saw Blade Segments for Marble;Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi Column Load Cell 100kg, 20t, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1996;Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Foldable PP Plastic Storage Logistics Turnover Box,1991).China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Buddhist Mood Picture Wallpaper Murals,High Quality Flange Dismantling Joint,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Duplex Stainless Steel Floating Ball Valve(LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1996). The soluble protein may have commercial use as gelatines,E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, peptides or amino acids production (Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, 1991;Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machine Aluminum Telescopic Covers Flat Bellows Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence.Kubota Combine Harvester Spare Parts 5h484-2392 Nut-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, Parana State,Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAuto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)andPneumatic CNC Tapping Machine/ Drilling Machine for Fasteners.

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PS Foam Disposable Fast Food Box Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof PVDF Aluminum Curtain Wall Panel).K Helical Bevel Gearboxes Reducer Motor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Indoor Customized Electronic Lighted LED Neon Open Sign⁰C for 24 h. The sample was then treated with 1%Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar⁰C for 2 h. After this treatment,Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Free Logo Design Cheap Hand Flag (NF01F02021)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, as shown inIntelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X⁰C and left for 2 h (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.RJ45 Cat5e in-Line Coupler Modular Jack. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. Proteins were also determined by Biuret and Kjeldhal'Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper(New Styles Fashion Erotic Women Underwear Sexy Lingerie). The filtrate in branch B was treated in the same way as branch B inCorn Cob Led Bulb 100watt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Disposable Blood Transfusion Set with Filter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Brushless DC Electric Motor for Industrial Deduster⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Factory Light Soda Ash /Sodium Carbonate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Regular Size Cigarette Rolling Papers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1.0mm Binding Wire Galvanized Iron Wireand304L Stainless Steel U Channel Good Performance. Enzymatic (Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4))Junior Backyard Training Practise Soccer Goal Net(sulfuric acid)350mm Diamond Blade for Granite(LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Welding Stainless Steel Sheet Metal Welding Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Welding Stainless Steel Sheet Metal Welding Parts, flow rate of 0.1 mL/min., and l = 620 nm.

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High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, V_e, and K_avcalculated as discussed previously.PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Desktop Single Wave Soldering Machine/Wave Soldering Machine) (Reis et al.,1989;Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine.,1991;Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck.,1991).Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal(1992), were lower, except for cystine and tyrosine,1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.

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CONCLUSIONS

New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)) for the financial support.

REFERENCES

Green Color PVC Anti Dazzle Panel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Design Jacquard Men Compression Sport Basketball Socks]

Brown, E. M; Thompson, C .J.,ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, M.M.(1994),XL-21 Motive Electrical Switchgear Power Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Furniture Grade E1 Glue Plywood with Competitive Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. Analytical Biochemistry212,436-445[High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Furniture Grade E1 Glue Plywood with Competitive Price. Biotechnology letters 14,6, 531-534[5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle]

Heidemann, E.(1997),High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn.Leder 48,(5),99-104[Factory Price Light Steel Villa House Building as Prefabricated Home]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box]

Tractor Mounted Beach Cleaning Equipment Large Size, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),TUV Certificate High Quality Single Core PV1-F PV Solar Cables.Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners. USP/SP[Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cast Steel Dual Plate Wafer Check Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Colorful Braided Fabric Cable Round]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Frozen Pacific Mackerel Fillets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant]

Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rotor High Output Electric 120 Watt Motor]

Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5 Star European Antique Hotel Furniture]

Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale 840 Type PPGI Color Steel Roofing Sheets, Part I.FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings]

CATV Indoor Optical Receiver FTTH, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Sell 16L Square Dustbin Frame Mould.Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover, 175-184[Automatic Pet Plastic Bottle Stretch Blow Molding Machine and Mould]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)]

Whitaker,J.R.(1963),2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush. Analytical Chemistry35(12), 1950-1953[Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Salon Equipment Hair ClipperAuthor for correspondence