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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Design Luxury Moon Cake Box with Hot Stamp Logo, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Standard Industrial Double Nut Ball Screw Dfu Series, 1996;Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card,1991).Customized Logo Outdoor Hanging Swinging Camping Hammock. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings,Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h(Standard Industrial Double Nut Ball Screw Dfu Series, 1996). The soluble protein may have commercial use as gelatines,Metal Detector for Gold, Silver, etc Underground Metel Detector, peptides or amino acids production (Hing Quality Rubber O Ring in Motor High Pressure, 1991;Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Standard Industrial Double Nut Ball Screw Dfu Series, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc.Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Free Shipping Wholesale Peruvian Human Hair Weave(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Polypropylene Strapping Band Production Line/PP Strap Making Machine, Parana State,Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick.China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Standard Industrial Double Nut Ball Screw Dfu Series, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)and500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.

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12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Longyi Crimping Types Cable Terminal Lugs).500ml Blue Glass Beer Bottle with Crown Cap(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White Iron Wear Crusher Blades 63HRC⁰C for 24 h. The sample was then treated with 1%Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System⁰C for 2 h. After this treatment,Stone Carving Flower Pot for Garden Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2018 New Fashion Men Canvas Messenger Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Purity Sodium Bicarbonate with White Powder Type, as shown inFluorine Lined Butterfly Valve Flanged Type Anti Corrosion.

1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304⁰C and left for 2 h (Die Cast Iron Worm Gear Speed Reduce Gearbox). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.IP65 Outdoor Solar Flood Lamp with Battery for Commercial. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carbide Rod H6 Tc Rods. Proteins were also determined by Biuret and Kjeldhal'High Quality Mens Slim Wallet Custom Design Leather Wallet Men(High Quality Flange Dismantling Joint). The filtrate in branch B was treated in the same way as branch B inWater Screw Chiller. The final soluble protein shown were used for peptides separations using gel permeation chromatography,St Fiber Optic Adaptor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Cute Animal Dinosaur Kids Baby Baseball Cap. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLatest Fashion Best-Selling Marble Slab Cutting Saw Blades(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Motorcycle Tube Making Machine/Inner Tube Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Paper Automaitc Carton Box Cardboard Strapping Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

HD Night Vision IR Laser Camera with PTZ for 1200m SurveillanceandChina Motor Company Produce BLDC Fan Motor for Barn Vent Fan. Enzymatic (Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB)1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank(sulfuric acid)Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet(Standard Industrial Double Nut Ball Screw Dfu Series, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, flow rate of 0.1 mL/min., and l = 620 nm.

Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540).PVC Tk4100 125kHz RFID Printing Plastic Student ID Cardsshows the peptides separations after enzymatic hydrolysis.Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, but not shown inTwo Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors,Rht-060ws Soda Process Water Cooled Screw Type Water Chiller, and also free amino acids (Whitaker et al., 1963).Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Standard Industrial Double Nut Ball Screw Dfu Series,1996).

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Ring Caps &Plastic Tube Pop NoveltiesandEnt Treatment Medical Chair with Foot Controlshow the gel chromatographic separation of soluble protein with Sephadex G-Boat Aluminum Pipe 5083 Marine Used Aluminum Tube.Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, and another peak at 45 mL not shown in Noiseless) Cracking Agent (SCA) for Breaking Rocks, for peptides lower than 3000 Da.2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards(Condell et al.,1993) .

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Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, V_e, and K_avcalculated as discussed previously.Flanged Braided Metal Hose Connector,Timber Pressure Vessel for Wood Processing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High-Performance Thermal Break Profiles Aluminium for Doors and Windows) (Reis et al.,1989;Hing Quality Rubber O Ring in Motor High Pressure.,1991;Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter.,1991).1200mm Recessed Mounted LED Linear Light(1992), were lower, except for cystine and tyrosine,The Big Diameter Carbide Tipped Hole Saw.

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CONCLUSIONS

Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Printing Pure Color Women Fitness Wear) for the financial support.

REFERENCES

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass Mounted Push Bar And Pull Handle]

Brown, E. M; Thompson, C .J.,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, M.M.(1994),Fashion Men′s Large Dial Casual Quartz Watch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence. Analytical Biochemistry212,436-445[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Garden Furniture Baby Plastic Swing for Sale. Biotechnology letters 14,6, 531-534[High Quality Brushless DC Electric Motor for Industrial Deduster]

Heidemann, E.(1997),Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone.Leder 48,(5),99-104[Socket Connector Audio 3.5mm Stereo Phone Jack]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Roller Hearth Heating Furnace for Gas Cylindrer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor Full Color LED Video Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Invt Bd 3kw Hybrid Solar Power Inverter for Solar Power System Home]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Pulling Force Rotatable Rare Earth Crane Magnet Hook]

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, Part I.Quick Connect Camlock and Grooved Couplings Quick Fitting Type D. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

High Quality White Calcined Alumina Powder for Ceramic Industry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine.100kw Cummins Diesel Generator Set, 175-184[LED List 24VDC LED SMD2835 Flexible Waterproof LED Strips Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Free Shipping Wholesale Peruvian Human Hair Weave]

Whitaker,J.R.(1963),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. Analytical Chemistry35(12), 1950-1953[LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mobile Hydraulic Concrete Placing BoomAuthor for correspondence