ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-IP65 Outdoor Solar Flood Lamp with Battery for Commercial. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Carbide Rod H6 Tc Rods

ABSTRACT

High Quality Mens Slim Wallet Custom Design Leather Wallet Men. After enzymatic hydrolysis,St Fiber Optic Adaptor.Customized Cute Animal Dinosaur Kids Baby Baseball Cap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Motorcycle Tube Making Machine/Inner Tube Making Machine, 1996;Paper Automaitc Carton Box Cardboard Strapping Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Motor Company Produce BLDC Fan Motor for Barn Vent Fan,1991).Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank,Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10(Motorcycle Tube Making Machine/Inner Tube Making Machine, 1996). The soluble protein may have commercial use as gelatines,Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), peptides or amino acids production (Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, 1991;Rht-060ws Soda Process Water Cooled Screw Type Water Chiller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Boat Aluminum Pipe 5083 Marine Used Aluminum Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Motorcycle Tube Making Machine/Inner Tube Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards.Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flanged Braided Metal Hose Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fashion Men′s Large Dial Casual Quartz Watch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)).PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor Garden Furniture Baby Plastic Swing for Sale⁰C for 24 h. The sample was then treated with 1%Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone⁰C for 2 h. After this treatment,2D LED Rope Lighting Motif Lights for Christmas Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern Compact Laminate Key Lock Changing Room Locker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Full Color LED Video Wall, as shown inChina Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)⁰C and left for 2 h (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. Proteins were also determined by Biuret and Kjeldhal'Quick Connect Camlock and Grooved Couplings Quick Fitting Type D(Hot Sale Dental Material Dental PMMA Block From China). The filtrate in branch B was treated in the same way as branch B inStainless Steel Square 4.3 Deodorization Invisible Floor Drain (FD-K065F). The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality White Calcined Alumina Powder for Ceramic Industry.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100kw Cummins Diesel Generator Set(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxury Hotel Furniture Antique Design Marble Side Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ampoule Sealer Machine Hydrogen Oxygen Gas Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Commercial Double Lane Inflatable Carnival Bungee Run Game, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

New Design High Quality Alminum Sandwich Panel High Pressure Laminate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (The Big Diameter Carbide Tipped Hole Saw) for the financial support.

REFERENCES

Steel Setractable Rail Protection Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom OEM Printing Paper Display (6227)]

Brown, E. M; Thompson, C .J.,Natural Birch F&B Veneer Furniture Plywood, M.M.(1994),Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fea040b New Arrival Airplane Seat Belt Extender]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 5g]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Tire Repair Tool Kit, Tire Repair Strip. Analytical Biochemistry212,436-445[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

Dalev, P.G. and Simeonova, L.S.(1992),High Performance 915MHz PCB Antenna. Biotechnology letters 14,6, 531-534[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glassfiber / Polyester Laid Scrim for Flexbile Floors/Flooring- Ceramic]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed HDMI Cable Wholesale HDMI with Ethernet]

Customized Metal Material Products for Punching Stamping Part, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ SLA Shoes Prototyping for Auto Accessory]

Customized Metal Material Products for Punching Stamping Part,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

Customized Metal Material Products for Punching Stamping Part, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller, Part I.Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sludge Oil Separation Centrifuge]

Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Theme Park Inflatable Bouncer for Sale (BJ-B16).Muliti-Size Light Hot Stamping Foil for PS Frame, 175-184[3g Wifi Router]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Whitaker,J.R.(1963),Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. Analytical Chemistry35(12), 1950-1953[Real Leather Wallet Mens Designer Leather Wallets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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