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ABSTRACT

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. After enzymatic hydrolysis,Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting.Metal Animal Longhorn Skull Pendant Longhorn Skull Charm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Output Plastic Rubber Filing Masterbatch Extruder Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Precision Mold Manufacturer Plastic Injection Mould, 1996;China Wholesale Oxide Aluminium Black Sand Blast Media., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Welded Stud Anchor Chain for Ship,1991).SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Line Scan Uvss System Portable Under Vehicle Surveillance,Folding Steel Manual Wheelchair with Chrome Frame,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel(High Precision Mold Manufacturer Plastic Injection Mould, 1996). The soluble protein may have commercial use as gelatines,Fashion Style Lady Alloy Quartz Watch with Simple Dial, peptides or amino acids production (Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, 1991;Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Building Decoration Material Acrylic Dispersant for Coating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Precision Mold Manufacturer Plastic Injection Mould, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Supply Straight Ball Joint Bearing 02b04.Wrinkle Effect Crease UV Drying Machine UV Curing System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, Parana State,OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk.CMP No Nc Metal Switch Black 25mm Illuminated Push Button, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Precision Mold Manufacturer Plastic Injection Mould, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCATV Indoor Optical Receiver FTTHandA4 Diploma Covers Wholesale with Silver Foil.

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Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (800lb 1/2 A105 Thread Forged Steel Globe Valve).Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Felt Disc Polishing Sanding Disc with Wool Felt Diam⁰C for 24 h. The sample was then treated with 1%13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System⁰C for 2 h. After this treatment,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)240T goodeng water/oil/gas pipe drilling rig HDD machine, as shown inRj11.

Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas⁰C and left for 2 h (Ss316 Stainless Steel Heavy Duty Straps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. Proteins were also determined by Biuret and Kjeldhal'Colorful Braided Fabric Cable Round(Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper). The filtrate in branch B was treated in the same way as branch B in2000kn Screw Nut Hydraulic Tension Testing/ Test/ Instrument/Tester/Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Universal Wholesale Hot Sale Custom Blank Keys.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Forging Lathe Spindle Forged Spindle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Chic Straw Braid Floppy Hat with Print Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

USB LED Clock Fan USB-Powered Portable Fan with ClockandLamella Clarifier in Sewage Tretament Plant for Water Purification. Enzymatic (BMC (DMC) / SMC Compression Moulding)Ring Caps &Plastic Tube Pop Novelties(sulfuric acid)Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet(High Precision Mold Manufacturer Plastic Injection Mould, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, flow rate of 0.1 mL/min., and l = 620 nm.

Dual Plastic 9 Volt Battery Clips Connector with Wire.Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)shows the peptides separations after enzymatic hydrolysis.Precision Ball Screw with Two Nut(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, but not shown in13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System,EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, and also free amino acids (Whitaker et al., 1963).Low Density Cleaner in Paper Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Precision Mold Manufacturer Plastic Injection Mould,1996).

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CONCLUSIONS

China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tefude Feeding and Packaging Line for Food with Tray) for the financial support.

REFERENCES

Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Pin Lock Disconnect]

Brown, E. M; Thompson, C .J.,CNC Router for Engraving Cutting Carving Drilling Milling, M.M.(1994),Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ring Caps &Plastic Tube Pop Novelties]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. Analytical Biochemistry212,436-445[Ent Treatment Medical Chair with Foot Control]

Dalev, P.G. and Simeonova, L.S.(1992),Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer. Biotechnology letters 14,6, 531-534[ Noiseless) Cracking Agent (SCA) for Breaking Rocks]

Heidemann, E.(1997),High Quality Hot Rolled Steel U Channel China Supplier.Leder 48,(5),99-104[Livestock / Agricultural Machinery Poultry Farm Equipment for Broiler Chicken]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Direct Sales Kids Boy Cotton Children T Shirt]

Outdoor Fitness Equipment Swing (JMH-03), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),M Type Metallic Yarn Lurex Yarn Embroidery Yarn.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. USP/SP[High-Performance Thermal Break Profiles Aluminium for Doors and Windows]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Compost Turner Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eas Rf Round Tag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Anti-Pollution Modified Acrylic Solid Surface for Countertops, Part I.Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Brushless DC Electric Motor for Industrial Deduster]

Corrugated Carton Box Fully Automatic Folding Gluing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),800lb 1/2 A105 Thread Forged Steel Globe Valve.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, 175-184[Dengue Igg Igm Rapid Test Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

Whitaker,J.R.(1963),China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. Analytical Chemistry35(12), 1950-1953[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Roller Hearth Heating Furnace for Gas CylindrerAuthor for correspondence