ARTICLES

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ABSTRACT

20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. After enzymatic hydrolysis,8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush.Hybrid Inverter Combined MPPT Controller 1-12kw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery;Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Newest Men Watches Popular Products Mens Custom Wood Watch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Accurate Measuring Rulers 3D Lenticular Plastic Rulers, 1996;Reusable PLA and Pbat Tea Packing Pouch Supplier in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry,1991).Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial P-Toluene Sulfonic Acid for 93%-99% Purity,Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair(Accurate Measuring Rulers 3D Lenticular Plastic Rulers, 1996). The soluble protein may have commercial use as gelatines,High Purity99.95% Cobalt Oxide, peptides or amino acids production (Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 1991;Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Accurate Measuring Rulers 3D Lenticular Plastic Rulers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated.6000 Series Anodizing Extrusion Aluminum Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100WAlcalase2.5 L DX,Tomato Paste Making Machine-Turn Key Solution, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

HPMC Similiar to Tylose Powder for Rapid-Set Adhesive, Parana State,High-Tower Compound NPK Fertilizer 15-15-15.NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Accurate Measuring Rulers 3D Lenticular Plastic Rulers, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery PackandHot Selling Knitting Velvet Jaguar Fabric (JAGUAR).

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a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy).Hot Selling Solidwood Viola Wholesale Price Viola(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Building Material Sound Heat Insulation Rock Wool Sandwich Panel⁰C for 24 h. The sample was then treated with 1%Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration⁰C for 2 h. After this treatment,Ltmg Classic Product 6 Ton Wheel Front Loader for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar, as shown inStainless Steel DIN7981 Pan Head Self Tapping Screw.

Samsung LTI550HN16 1920X1080P monitor screen LCD video wall⁰C and left for 2 h (6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Household Family Medical Emergency Electronic Digital Thermometer. Proteins were also determined by Biuret and Kjeldhal'Die Cast Iron Worm Gear Speed Reduce Gearbox(New Arrival Plush Animal Ride for Kids for Shopping Center). The filtrate in branch B was treated in the same way as branch B inStainless Steel AISI316/304 Spring Snap Hook with Eyelet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Couple Watches Steel Material Gift Watch OEM in China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlass Tea Sugar Coffee Storage Jar with Screw Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Particle Board Manufacturer, Melamine/Plain Chipboard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

No 55 Steel Wire Cross Type Razor Barbed WireandVilla Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G). Enzymatic (Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert)Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps(sulfuric acid)120ml Glass Perfume Bottles for Designer Perfume(Accurate Measuring Rulers 3D Lenticular Plastic Rulers, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Agriculture Hay Silage Wrapping Film, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Agriculture Hay Silage Wrapping Film, flow rate of 0.1 mL/min., and l = 620 nm.

Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.Indoor Load Break Switch Isolating Switchshows the peptides separations after enzymatic hydrolysis.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSTPP Sodium Tripolyphosphate for Detergent / Ceramic / Food, but not shown inNordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate,16oz Insulated Cardboad Cup Sleeve, and also free amino acids (Whitaker et al., 1963).Large Outdoor Simple Gazebo Beer Festival Marquee Tent. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Accurate Measuring Rulers 3D Lenticular Plastic Rulers,1996).

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T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, V_e, and K_avcalculated as discussed previously.LED Rectangular Recessed Linear Lamps for Bank and Car Sale,Salon Equipment Hair Clipper. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment) (Reis et al.,1989;Wood Activated Carbon for Decolorizing in Food Industry with ASTM.,1991;Reusable PLA and Pbat Tea Packing Pouch Supplier in China.,1991).Flat Conveyor Belt Steel Idler Frame for Roller(1992), were lower, except for cystine and tyrosine,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6.

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CONCLUSIONS

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper) for the financial support.

REFERENCES

Durable TPR Pet Molar Toy with Colorful Rope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Neodymium Magnet]

Brown, E. M; Thompson, C .J.,Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, M.M.(1994),Chromium Oxide Green 99% for Construction and Plastics.The Journal of the American Leather Chemists Association (JALCA)89(7)[Q3210 Vibratory Polishing Shot Blasting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Building Decoration Material Acrylic Dispersant for Coating]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Zs Round Vibrating Sieve. Analytical Biochemistry212,436-445[Line Scan Uvss System Portable Under Vehicle Surveillance]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Tactile Paving Blind Road Stud. Biotechnology letters 14,6, 531-534[Customized Logo Outdoor Hanging Swinging Camping Hammock]

Heidemann, E.(1997),70L Lab Use Refrigerated Circulator Chiller Factory Price.Leder 48,(5),99-104[www.lingkebags.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

120L 240L Oil Universal Chemical Spil Absorbent Kits, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).Roller Hearth Heating Furnace for Gas Cylindrer. USP/SP[Factory Supply Straight Ball Joint Bearing 02b04]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-custom design MIFARE DESFire EV2 2K plastic RFID loyalty card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High-Tower Compound NPK Fertilizer 15-15-15]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hscan771 3D Scanner High Resolution 3D Remolding Device - Scantech]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4.0MP Ahd Tvi CVBS HD CCTV Camera Module]

Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hotel Card Door Lock Access Control Safe Door Lock-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Precision Ball Screw with Two Nut]

Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wood Fireplace Double Skin Chimney Flue Pipes]

Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, Part I.Stainless Steel DIN7981 Pan Head Self Tapping Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch]

19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.Dark Green Decorative Garden Welded Wire Mesh Fencing, 175-184[40X-1600X Biological Microscope Xsz-107V LED Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Screw Machine]

Whitaker,J.R.(1963),Csm DIY Microscope for Kids/Toy Microscope. Analytical Chemistry35(12), 1950-1953[5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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