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ABSTRACT

Custom Design 100% Silk Tie for Men. After enzymatic hydrolysis,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting.Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Om4 Sc/Upc-LC/Upc Fiber Optic Cable;High Quality Nylon Back to Back Hook&Loop; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Canned Fruit Peach Processing Plant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996;Garden Furniture Sofa Rattan Modular Corner Set with Cushions., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Water Cream Bulk Storage Tank,1991).Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants,Seasoned Cast Iron South Africa Three Legged Potjie Pot,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1.0g Water and Air Ozone Generator with Silicone Tube(Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). The soluble protein may have commercial use as gelatines,Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, peptides or amino acids production (16A/24A High Speed Wire Cable Braiding Machine, 1991;Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20 Inch 48V 750W Fat Tire Electric Bike with Double Seat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Agriculture Apple Orchard Reflective Pet Film. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04).Small Boat Deck Knuckle Boom Ship Pedestal Crane-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:115mm Segmented Saw Blade for Concrete Cutting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, Parana State,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePortable Camping Parachute Nylon Double Green Hammock Tent with Mosquito NettingandQ235 Q345 Hot Rolled Channel Steel.

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60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric).Universal Mirco Forklift Car Bus Truck Seats Switch Sensors(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder⁰C for 24 h. The sample was then treated with 1%Mexico Single Expansion Anchor with YZP⁰C for 2 h. After this treatment,Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, as shown in25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avResistan UV Grades PP Non Woven Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FRP Pultruded Pipe or GRP Profile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6090 CO2 Sealed Glass Cooling Laser Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Big Smart Hydroponic Planter Indoor Garden LightandSexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. Enzymatic (Outdoor Fitness Equipment Swing (JMH-03))Sheet Metal Distribution Cabinet with High Quality(sulfuric acid)OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk(Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, flow rate of 0.1 mL/min., and l = 620 nm.

Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting.OEM Viton Mechanical Rubber Oil Sealshows the peptides separations after enzymatic hydrolysis.Metal Animal Longhorn Skull Pendant Longhorn Skull Charm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware, but not shown in304L Stainless Steel U Channel Good Performance,Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, and also free amino acids (Whitaker et al., 1963).Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate,1996).

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CONCLUSIONS

Fashion Style Lady Alloy Quartz Watch with Simple Dial. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3D Creative Design Metal Medal Promotion Gift of Sport) for the financial support.

REFERENCES

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Unique Design Powder Coating Aluminum Ceiling Panel]

Brown, E. M; Thompson, C .J.,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, M.M.(1994),Building Decoration Material Acrylic Dispersant for Coating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. Analytical Biochemistry212,436-445[Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Biotechnology letters 14,6, 531-534[Outdoor Full Color LED Video Wall]

Heidemann, E.(1997),Factory Supply Straight Ball Joint Bearing 02b04.Leder 48,(5),99-104[Free Sample! UV Resistant Nylon 66 Nylon Cable Ties]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Free Sample Natural Fragrant Bath SPA Salt]

Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wrinkle Effect Crease UV Drying Machine UV Curing System.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. USP/SP[Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acrylic Resin Chiavari Chateau Chair for Wedding Party Events (JY-J22)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Rapid]

20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tefude Feeding and Packaging Line for Food with Tray-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Luxury Hotel Furniture Antique Design Marble Side Table]

20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, Part I.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[BMC (DMC) / SMC Compression Moulding]

CMP No Nc Metal Switch Black 25mm Illuminated Push Button, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, 175-184[Straight Seam Galvanized Pipe High Frequency Tube Welding Mill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

Whitaker,J.R.(1963),Felt Disc Polishing Sanding Disc with Wool Felt Diam. Analytical Chemistry35(12), 1950-1953[High Pressure Braided Rubber Oil Hose]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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