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ABSTRACT

16A/24A High Speed Wire Cable Braiding Machine. After enzymatic hydrolysis,Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot.5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Boat Deck Knuckle Boom Ship Pedestal Crane, 1996;115mm Segmented Saw Blade for Concrete Cutting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit,1991).China Round Transparent Glass 750ml Liquor Glass Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D Creative Design Metal Medal Promotion Gift of Sport,Ad-L13 Black Perfume Plastics Vial Bottle 10ml,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(Small Boat Deck Knuckle Boom Ship Pedestal Crane, 1996). The soluble protein may have commercial use as gelatines,EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), peptides or amino acids production (60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light, 1991;Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mexico Single Expansion Anchor with YZP.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Boat Deck Knuckle Boom Ship Pedestal Crane, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet.Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Whole Absorbtion Flexo Printing Soltting Die Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Output Plastic Rubber Filing Masterbatch Extruder Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Wholesale Oxide Aluminium Black Sand Blast Media⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Welded Stud Anchor Chain for Ship, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

CMP No Nc Metal Switch Black 25mm Illuminated Push Button. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Cmyk Printing Cake Gift Packing Box with Lid) for the financial support.

REFERENCES

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Four Color Printing PVC NFC Contact IC Card]

Brown, E. M; Thompson, C .J.,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, M.M.(1994),Felt Disc Polishing Sanding Disc with Wool Felt Diam.The Journal of the American Leather Chemists Association (JALCA)89(7)[100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. Analytical Biochemistry212,436-445[Custom Cmyk Printing Cake Gift Packing Box with Lid]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hotel Card Door Lock Access Control Safe Door Lock]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor Customized Electronic Lighted LED Neon Open Sign]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Accelerated Aging Oven Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

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Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Universal Wholesale Hot Sale Custom Blank Keys, Part I.Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Leaf Shape Pearl Jewelry Wedding Tiara]

Whitaker,J.R.(1963),Hot Sale Chic Straw Braid Floppy Hat with Print Tape. Analytical Chemistry35(12), 1950-1953[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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