ARTICLES

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ABSTRACT

Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. After enzymatic hydrolysis,Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck.500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Corrugated Carton Box Fully Automatic Folding Gluing Machine, 1996;800lb 1/2 A105 Thread Forged Steel Globe Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence,1991).China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining Metal Mechanical Engineering Parts,Low Sulphur Calcined Carbon Raiser,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Selling Free Sample Natural Fragrant Bath SPA Salt(Corrugated Carton Box Fully Automatic Folding Gluing Machine, 1996). The soluble protein may have commercial use as gelatines,Custom FSTN Va COB Cog Segment LCD Display Module, peptides or amino acids production (Jc680 High Temperature Pressure Transmitter, 1991;Deep Hole Cylinder Boring Machine for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling 202 Stainless Steel Coil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.NPK 16X Hammer Spare Parts Racing Forged Piston.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Corrugated Carton Box Fully Automatic Folding Gluing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.Mobile Hydraulic Concrete Placing Boom-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClear PVC Roof Frame Tent in South Africa(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Bend Pipe for Industrial Use⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ybz-E16f20 Vane Steering Hydraulic Pumps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Snow White Pumpkin Seeds for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM ABS Industrial Plastic Sheet Injection Auto Part.Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing Systemshows the peptides separations after enzymatic hydrolysis.15L Big Cooling Cylinder High Production Hard Ice Cream Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Port Small Automatic Electronic Plumbing Ball Valve, but not shown in20 Ton Small Vertical Plastic Injection Molding Machine,Flange Type Ball Float Water Tank Control Valve, and also free amino acids (Whitaker et al., 1963).925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Corrugated Carton Box Fully Automatic Folding Gluing Machine,1996).

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CONCLUSIONS

Gradient Print Overlay Push Button Membrane Keypad Switch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Cast Steel Flanged One Way Swing Check Valve Nrv) for the financial support.

REFERENCES

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Customized Metal Stamping Brace]

Brown, E. M; Thompson, C .J.,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), M.M.(1994),Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap CNC Spindle Peeling Lathe for Steel Bar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. Analytical Biochemistry212,436-445[Light Blue PVC Plastic Sheet and PVC Film]

Dalev, P.G. and Simeonova, L.S.(1992),AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle. Biotechnology letters 14,6, 531-534[Foldable PP Plastic Storage Logistics Turnover Box]

Heidemann, E.(1997),Low Power Consumption Silicone Sex Doll Vending Machine.Leder 48,(5),99-104[UL3239 AWG Awm DC 50kv Silicone High Voltage Wire Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer]

Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Toughened Glass Disc Suspension Insulator.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. USP/SP[Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[TM]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ATV Spiked Chain Harrow for Farming]

Customized Round Airless Lotion Pump Bottles for Skin Care Products, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Round Shape Dinner Football Gift Bowl with Cover Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1.8ml Clear Sample Vials with Label and PP Caps]

Customized Round Airless Lotion Pump Bottles for Skin Care Products,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay]

Customized Round Airless Lotion Pump Bottles for Skin Care Products, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Exporter of Wheat Flour Mill Plant (80t), Part I.ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 New Water Quality TDS Test]

Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Alloy Rack for Conductor, Wire, Cable.Outdoor Accessories Fishing Hunting Masks, 175-184[5m Hirose M12 6pin 12pin Power and Io Cable with Flying Leads for Analog Camera Connection]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

Whitaker,J.R.(1963),Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. Analytical Chemistry35(12), 1950-1953[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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