ARTICLES

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ABSTRACT

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. After enzymatic hydrolysis,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.ASTM A240 304 316L No. 1 Stainless Steel Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.25kg Fertilizer Belt Packing Machine for Special Materials, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, 1996;Rotor High Output Electric 120 Watt Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby,1991).Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine,Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rpl Straight Socket and Plugs Male Thread(Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, 1996). The soluble protein may have commercial use as gelatines,46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, peptides or amino acids production (OEM Metal Stamping Stainless Steel Parts with CNC Machining, 1991;Ss Wafer Type Check Valve Dn32 Dn40 Dn50, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰WPC Wood Plastic Board Equipment Extruder Machine.Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Neoprene Elbow Support(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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275ml Frosted Clear Glass Drinking Bottle for Juice(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine).EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs⁰C for 24 h. The sample was then treated with 1%Bolts and Nuts Set Brass Fasteners Double Hex Bolt⁰C for 2 h. After this treatment,Factory Direct Sales Kids Boy Cotton Children T Shirt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, as shown ina PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine.

Light Blue PVC Plastic Sheet and PVC Film⁰C and left for 2 h (Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metric Female Cone Hydraulic Hose Fitting. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Dental Material Dental PMMA Block From China(Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema). The filtrate in branch B was treated in the same way as branch B inm.macwellwheel.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Waste Pet Flakes Recycling Washing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Deposited Stainless Steel Rivet Nut with Knurled. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Stainless Steel Mechanical Filter Hosing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/BLE5.0 Ttl to Bluetooth Module with at Command, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cattle Corpse Cremation Burning Stove. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multi Sensor Nilitary Surveillance Thermal Imaging Security camera) for the financial support.

REFERENCES

L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[C240 Isuzu Diesel Engine Parts]

Brown, E. M; Thompson, C .J.,E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge, M.M.(1994),2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Round Airless Lotion Pump Bottles for Skin Care Products]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Stainless Steel Biological Fermentor. Analytical Biochemistry212,436-445[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

Dalev, P.G. and Simeonova, L.S.(1992),16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive. Biotechnology letters 14,6, 531-534[100% Latex Free Endotracheal Tube with PVC Material]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Solar Powered Outdoor Lighting LED Solar Wall Light]

Cleanroom White PVC Dotted Anti-Static Gloves, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part.16 Spindle Lace High Speed Braiding Machine Textile Machinery. USP/SP[Latest Lace Fabric Design Logo Flag Embroidery Patch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Micro Button Type Load Cell Compression Load Cell 10kg: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Waste Pet Flakes Recycling Washing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8011 Pharmaceutical Aluminum Foil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Beans]

Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Interlocking Precast Plastic Concrete Paver Molds for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, Part I.900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Flange Housing Ucf310-115 for Electric Hand Tools.Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors, 175-184[China Automatic Multifunction Vertical Type Back Sealing Granule Packing Machine for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

Whitaker,J.R.(1963),Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery. Analytical Chemistry35(12), 1950-1953[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Produce Medical Machining Custom Metal MillingAuthor for correspondence