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ABSTRACT

Water Chiller Air Cooled for Cooling Beer Refrigerating. After enzymatic hydrolysis,High Quality Cheap Price Disposable IV Infusion Set with Precise Filter.Q3210 Vibratory Polishing Shot Blasting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Design Pet Product, Climbing Willow Cat Scratcher Tree, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine, 1996;Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide,1991).Single and Multi Rope Wire Mine Hoist From China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine,Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Electronic Circuit Board Supplier PCB Assembly(Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine, 1996). The soluble protein may have commercial use as gelatines,Femo Ferro Molybdenum Powder Lump 55/60, peptides or amino acids production (8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight, 1991;Round Panoramic Lift, Glass Observation Passenger Elevator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Painting Road Marking Vehicles Machine for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automotive Rubber Parts for Any Rubber Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Die Casting Zinc Alloy Badge Use for Car. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge.CREE Chip 24W 28W 30W LED Downlight-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale 840 Type PPGI Color Steel Roofing Sheets).Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mashroom Head Nylon Anchor with Pozi Nail⁰C for 24 h. The sample was then treated with 1%Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool⁰C for 2 h. After this treatment,Waterjet 87K Intensifier Part Check Valve Body 020077-1. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Meat Machinery/Chicken Breast and Fish Slicing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, as shown inYbz-E16f20 Vane Steering Hydraulic Pumps.

1.0mm Binding Wire Galvanized Iron Wire⁰C and left for 2 h (Colored Silicone NBR O-Rings Seals). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesales Stackable Coffee Iron Chair. Proteins were also determined by Biuret and Kjeldhal'OEM Eye Anti-Aging Serum(Electronic Foldable for Patient Treatment Ent Unit Chair). The filtrate in branch B was treated in the same way as branch B inPortable Hot Fix Gemstone Tools Vacuum Rhinestone Applicator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMicrofiber Towel Car Drying Soft Towel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Lathe Main Parts with Good Quality) for the financial support.

REFERENCES

Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Evaporation Equipment]

Brown, E. M; Thompson, C .J.,120L 240L Oil Universal Chemical Spil Absorbent Kits, M.M.(1994),Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High-Tower Compound NPK Fertilizer 15-15-15. Analytical Biochemistry212,436-445[HOWO Tipper Truck Price with Manufacture Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. Biotechnology letters 14,6, 531-534[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Square Shape Glass Candle Holder for One Piece Tealight Candle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Manufacture Salt Grinder with Ce]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Port Small Automatic Electronic Plumbing Ball Valve]

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Newest Men Watches Popular Products Mens Custom Wood Watch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[The Big Diameter Carbide Tipped Hole Saw]

Newest Men Watches Popular Products Mens Custom Wood Watch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reusable PLA and Pbat Tea Packing Pouch Supplier in China, Part I.Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial P-Toluene Sulfonic Acid for 93%-99% Purity.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, 175-184[Custom Printed Adhesive Security Warranty Void Packing Seal Tape]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Whitaker,J.R.(1963),Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair. Analytical Chemistry35(12), 1950-1953[NPK+Te Fertilizer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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