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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Factory Light Soda Ash /Sodium Carbonate. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Regular Size Cigarette Rolling Papers

ABSTRACT

304L Stainless Steel U Channel Good Performance. After enzymatic hydrolysis,Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).Junior Backyard Training Practise Soccer Goal Net. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;350mm Diamond Blade for Granite;Custom Welding Stainless Steel Sheet Metal Welding Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Laser ID Card Rigid PET Printing Sheet, 1996;New Arrival Plush Animal Ride for Kids for Shopping Center., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine,1991).(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ss316 Stainless Steel Heavy Duty Straps,6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Temperature Side Opening AAC Autoclave, AAC Block Making Machine(Laser ID Card Rigid PET Printing Sheet, 1996). The soluble protein may have commercial use as gelatines,PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, peptides or amino acids production (Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, 1991;Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Green Color PVC Anti Dazzle Panel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Laser ID Card Rigid PET Printing Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.XL-21 Motive Electrical Switchgear Power Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cast Steel Dual Plate Wafer Check Valve, Parana State,Regular Size Cigarette Rolling Papers.Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Laser ID Card Rigid PET Printing Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soapand8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush.

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Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hing Quality Rubber O Ring in Motor High Pressure).Hot Sale 840 Type PPGI Color Steel Roofing Sheets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector⁰C for 24 h. The sample was then treated with 1%CATV Indoor Optical Receiver FTTH⁰C for 2 h. After this treatment,Best Sell 16L Square Dustbin Frame Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush, as shown inHotel Card Door Lock Access Control Safe Door Lock.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Pulling Force Rotatable Rare Earth Crane Magnet Hook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-Performance Thermal Break Profiles Aluminium for Doors and Windows⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Design Luxury Moon Cake Box with Hot Stamp Logo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Standard Industrial Double Nut Ball Screw Dfu Series, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, V_e, and K_avcalculated as discussed previously.Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom,Free Shipping Wholesale Peruvian Human Hair Weave. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Container House for Clinic or Hospital) (Reis et al.,1989;Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.,1991;New Arrival Plush Animal Ride for Kids for Shopping Center.,1991).Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags(1992), were lower, except for cystine and tyrosine,Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter.

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CONCLUSIONS

Printing Pure Color Women Fitness Wear. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (TUV Certificate High Quality Single Core PV1-F PV Solar Cables) for the financial support.

REFERENCES

Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anti-Slip PVC/TPU Floor Paving Tactile Tile]

Brown, E. M; Thompson, C .J.,Polypropylene Strapping Band Production Line/PP Strap Making Machine, M.M.(1994),China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Competitive Price Sodium Gluconate as Concrete Admixture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B. Analytical Biochemistry212,436-445[Cr1000 Spray Nozzle Common Rail Injector Tester]

Dalev, P.G. and Simeonova, L.S.(1992),500ml Blue Glass Beer Bottle with Crown Cap. Biotechnology letters 14,6, 531-534[24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine]

Heidemann, E.(1997),White Iron Wear Crusher Blades 63HRC.Leder 48,(5),99-104[Paper Making and Processing Machine for Toilet/Napkin/Tissue Paper Production Line]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Marble Child Statue for Garden]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2018 New Fashion Men Canvas Messenger Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Motorcycle Tube Making Machine/Inner Tube Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Baby Wet Wipes Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine]

High Purity Sodium Bicarbonate with White Powder Type, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed Steel Mill Roll with High Quality]

High Purity Sodium Bicarbonate with White Powder Type,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

High Purity Sodium Bicarbonate with White Powder Type, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IP65 Outdoor Solar Flood Lamp with Battery for Commercial, Part I.Carbide Rod H6 Tc Rods. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

High Quality Mens Slim Wallet Custom Design Leather Wallet Men, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),St Fiber Optic Adaptor.Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 175-184[m.zbaolang.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box]

Whitaker,J.R.(1963),Latest Fashion Best-Selling Marble Slab Cutting Saw Blades. Analytical Chemistry35(12), 1950-1953[Factory Price 4X6 Acrylic Magnetic Photo Frame]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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