ARTICLES

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ABSTRACT

Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). After enzymatic hydrolysis,High Quality Brushless DC Electric Motor for Industrial Deduster.China Factory Light Soda Ash /Sodium Carbonate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Regular Size Cigarette Rolling Papers;304L Stainless Steel U Channel Good Performance; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Junior Backyard Training Practise Soccer Goal Net, 1996;350mm Diamond Blade for Granite., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Welding Stainless Steel Sheet Metal Welding Parts,1991).Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Laser ID Card Rigid PET Printing Sheet,New Arrival Plush Animal Ride for Kids for Shopping Center,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine(Junior Backyard Training Practise Soccer Goal Net, 1996). The soluble protein may have commercial use as gelatines,(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, peptides or amino acids production (Ss316 Stainless Steel Heavy Duty Straps, 1991;6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Temperature Side Opening AAC Autoclave, AAC Block Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Junior Backyard Training Practise Soccer Goal Net, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Furniture Grade E1 Glue Plywood with Competitive Price, Parana State,DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle.High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Junior Backyard Training Practise Soccer Goal Net, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion High Quality Head Layer Genuine Leather Men′s WalletandLinear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price.

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TUV Certificate High Quality Single Core PV1-F PV Solar Cables(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring).Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cast Steel Dual Plate Wafer Check Valve⁰C for 24 h. The sample was then treated with 1%Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products⁰C for 2 h. After this treatment,Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale 840 Type PPGI Color Steel Roofing Sheets's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, as shown inWaterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting.

CATV Indoor Optical Receiver FTTH⁰C and left for 2 h (New Design Pet Product). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Sell 16L Square Dustbin Frame Mould. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover. Proteins were also determined by Biuret and Kjeldhal'2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush(High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17). The filtrate in branch B was treated in the same way as branch B inBlack White Zigzag Full Printing Toilet Set 3 in 1 Nappa PU Cosmetic Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVDF Exterior Design Curved Aluminum Panel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNeodymium Magnet with RoHS ISO 16499 Approved(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Pulling Force Rotatable Rare Earth Crane Magnet Hook, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

120L 240L Oil Universal Chemical Spil Absorbent KitsandBlank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. Enzymatic (High-Performance Thermal Break Profiles Aluminium for Doors and Windows)Design Luxury Moon Cake Box with Hot Stamp Logo(sulfuric acid)Standard Industrial Double Nut Ball Screw Dfu Series(Junior Backyard Training Practise Soccer Goal Net, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, flow rate of 0.1 mL/min., and l = 620 nm.

Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card.Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950shows the peptides separations after enzymatic hydrolysis.Customized Logo Outdoor Hanging Swinging Camping Hammock(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1200X1000X810 Strong Collapsible Plastic Containers for Storage, but not shown inKt Board Acrylic CCD Camera CNC Knife Cutting Machine,EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings, and also free amino acids (Whitaker et al., 1963).Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Junior Backyard Training Practise Soccer Goal Net,1996).

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CONCLUSIONS

Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (XL-21 Motive Electrical Switchgear Power Cabinet) for the financial support.

REFERENCES

Free Shipping Wholesale Peruvian Human Hair Weave, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.meditecho2.com]

Brown, E. M; Thompson, C .J.,Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, M.M.(1994),Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Selling Custom Sheet Metal Fabrication]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Boat Deck Knuckle Boom Ship Pedestal Crane]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Printing Pure Color Women Fitness Wear. Analytical Biochemistry212,436-445[Double Sides Clear White Frosted Acrylic Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve. Biotechnology letters 14,6, 531-534[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

Heidemann, E.(1997),Polypropylene Strapping Band Production Line/PP Strap Making Machine.Leder 48,(5),99-104[Optical Night Sight Tool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B. USP/SP[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Roller Skating Sneakers Shoes for Children (8083)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500ml Blue Glass Beer Bottle with Crown Cap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Junior Backyard Training Practise Soccer Goal Net]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Screen Cleaning Rubber Ball]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

White Iron Wear Crusher Blades 63HRC, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Paper Automaitc Carton Box Cardboard Strapping Machine]

White Iron Wear Crusher Blades 63HRC,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cast Steel Dual Plate Wafer Check Valve]

White Iron Wear Crusher Blades 63HRC, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stone Carving Flower Pot for Garden Decoration, Part I.2018 New Fashion Men Canvas Messenger Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve]

High Purity Sodium Bicarbonate with White Powder Type, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304.IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 175-184[Saiding Auto Parts Water and Oil Separator for Toyota Land Cruiser 23380-17450]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[16A/24A High Speed Wire Cable Braiding Machine]

Whitaker,J.R.(1963),Carbide Rod H6 Tc Rods. Analytical Chemistry35(12), 1950-1953[Toughened Glass Disc Suspension Insulator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting MachineAuthor for correspondence