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ABSTRACT

120ml Glass Perfume Bottles for Designer Perfume. After enzymatic hydrolysis,Agriculture Hay Silage Wrapping Film.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane;16oz Insulated Cardboad Cup Sleeve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Large Outdoor Simple Gazebo Beer Festival Marquee Tent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HOWO Tipper Truck Price with Manufacture Factory Price, 1996;Wood Fireplace Double Skin Chimney Flue Pipes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality 304 Stainless Steel Sheet for Home Decoration Material,1991).T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Rectangular Recessed Linear Lamps for Bank and Car Sale,Salon Equipment Hair Clipper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Flat Conveyor Belt Steel Idler Frame for Roller(HOWO Tipper Truck Price with Manufacture Factory Price, 1996). The soluble protein may have commercial use as gelatines,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, peptides or amino acids production (Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, 1991;Durable TPR Pet Molar Toy with Colorful Rope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chromium Oxide Green 99% for Construction and Plastics.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Zs Round Vibrating Sieve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HOWO Tipper Truck Price with Manufacture Factory Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Tactile Paving Blind Road Stud.70L Lab Use Refrigerated Circulator Chiller Factory Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment).Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dark Green Decorative Garden Welded Wire Mesh Fencing⁰C for 24 h. The sample was then treated with 1%Csm DIY Microscope for Kids/Toy Microscope⁰C for 2 h. After this treatment,Oak Veneer Laminated Plywood for Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, as shown inMen Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses.

High Speed HDMI Cable Wholesale HDMI with Ethernet⁰C and left for 2 h (Roller Hearth Heating Furnace for Gas Cylindrer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Women′s Suit in Reasonable Price. Proteins were also determined by Biuret and Kjeldhal'Pet Short Fiber Non Woven Geotextile for Landfill Filtration(CMP No Nc Metal Switch Black 25mm Illuminated Push Button). The filtrate in branch B was treated in the same way as branch B inWarman Spares. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Competitive Price Sodium Gluconate as Concrete Admixture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrecision Investment Casting Stainless Steel 304 Wire Rope Hook(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Die Casting Aluminium Product for Connector Usage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Balustrade/Pillar/Column, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Pulling Force Rotatable Rare Earth Crane Magnet HookandPP/Pet Woven Geotextile for Road Construction. Enzymatic (Lamella Clarifier in Industrial Wastewater Treatment)FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering(sulfuric acid)Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED(HOWO Tipper Truck Price with Manufacture Factory Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, flow rate of 0.1 mL/min., and l = 620 nm.

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50g Body Luxury King Crown Cosmetic Containers, V_e, and K_avcalculated as discussed previously.Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men,Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New H Type Battery Cage System for Layer Chicken Farm) (Reis et al.,1989;Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.,1991;Wood Fireplace Double Skin Chimney Flue Pipes.,1991).Paper Egg Box Forming Molding Production Line/ Egg Tray Machine(1992), were lower, except for cystine and tyrosine,4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine.

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CONCLUSIONS

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016) for the financial support.

REFERENCES

CNC Metal Grinding Machine Tool Mk2120, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Astm A333 Gr6 Seamless Steel Pipe]

Brown, E. M; Thompson, C .J.,Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, M.M.(1994),Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Latest New Hho Engine Oil Carbon Remover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. Analytical Biochemistry212,436-445[Tefude Feeding and Packaging Line for Food with Tray]

Dalev, P.G. and Simeonova, L.S.(1992),12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. Biotechnology letters 14,6, 531-534[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Heidemann, E.(1997),Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.Leder 48,(5),99-104[Corner Sofa Set Designs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture]

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. USP/SP[2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Quality Hot Tube8 Japanese Stainless Steel Tube 666]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dg203 Medical Equipment Battery Power Home Using Electric Mobile Patient Transfer Lift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable]

N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Sale Various Customized Square 6063 Extruded Aluminum Profile]

N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed]

N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, Part I.High Quaity China Supplier Truck Trailer Auto Car Accessories. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light.Custom Steel Investment Casting High Hardness Agricultural Machinery Parts, 175-184[Chemical Agents High Quality Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

Whitaker,J.R.(1963),Minimalist Slim Real Leather Front Pocket Card Wallet. Analytical Chemistry35(12), 1950-1953[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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