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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Brand New Forklift with Double Front pneumatic Tire. After enzymatic hydrolysis,Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope.DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ce Quality Double Channel Medical Syringe Pump (THR-SP180D);SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Rubber Pressed Mixing Machine with Ce Certification, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, 1996;4K Giant Whiteboard Interactive Panel for University Whiteboard., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid,1991).Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting,Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Practical Edible Silicone Kitchenware (YB-ty-29)(Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, 1996). The soluble protein may have commercial use as gelatines,4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, peptides or amino acids production (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, 1991;Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Cube Ice Maker Parts / Ice Machines Maker Cube.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Adjustable American Market Cabinet Door Mini Hinge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Om4 Sc/Upc-LC/Upc Fiber Optic Cable, Parana State,Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips.High Quality Nylon Back to Back Hook&Loop, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRpl Straight Socket and Plugs Male ThreadandDeep Hole Cylinder Boring Machine for Sale.

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Canned Fruit Peach Processing Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flat Conveyor Belt Steel Idler Frame for Roller).Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Garden Furniture Sofa Rattan Modular Corner Set with Cushions⁰C for 24 h. The sample was then treated with 1%Stainless Steel Water Cream Bulk Storage Tank⁰C for 2 h. After this treatment,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Seasoned Cast Iron South Africa Three Legged Potjie Pot, as shown inOutdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.

1.0g Water and Air Ozone Generator with Silicone Tube⁰C and left for 2 h (500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,16A/24A High Speed Wire Cable Braiding Machine. Proteins were also determined by Biuret and Kjeldhal'Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot(Aws E7018 Low Carbon Steel Welding Electrode). The filtrate in branch B was treated in the same way as branch B inEverexceed 72V80A Pengiun SCR Single or Three Phase Motive / Intelligent/Industrial / Microprocessor / Forklift Battery Charger;. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20 Inch 48V 750W Fat Tire Electric Bike with Double Seat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAgriculture Apple Orchard Reflective Pet Film(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Boat Deck Knuckle Boom Ship Pedestal Crane⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/115mm Segmented Saw Blade for Concrete Cutting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Newest Men Watches Popular Products Mens Custom Wood WatchandChina Round Transparent Glass 750ml Liquor Glass Bottle. Enzymatic (3D Creative Design Metal Medal Promotion Gift of Sport)Ad-L13 Black Perfume Plastics Vial Bottle 10ml(sulfuric acid)Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), flow rate of 0.1 mL/min., and l = 620 nm.

60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light.Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Bladesshows the peptides separations after enzymatic hydrolysis.Universal Mirco Forklift Car Bus Truck Seats Switch Sensors(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLife Size 170cm Silicone Sex Dolls Real Love Doll for Male, but not shown inCustom Design 100% Silk Tie for Men,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, and also free amino acids (Whitaker et al., 1963).Mexico Single Expansion Anchor with YZP. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600,1996).

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Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bagand2PC Flanged&Threadedshow the gel chromatographic separation of soluble protein with Sephadex G-Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, and another peak at 45 mL not shown inGalvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, for peptides lower than 3000 Da.Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(Condell et al.,1993) .

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Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, V_e, and K_avcalculated as discussed previously.Factory Price 4X6 Acrylic Magnetic Photo Frame,200m Diesel Oil Hydraulic Water Well Drilling Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flanged Braided Metal Hose Connector) (Reis et al.,1989;High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine.,1991;4K Giant Whiteboard Interactive Panel for University Whiteboard.,1991).Custom Cmyk Printing Cake Gift Packing Box with Lid(1992), were lower, except for cystine and tyrosine,Air Purification Activated Alumina.

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CONCLUSIONS

Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting) for the financial support.

REFERENCES

Resistan UV Grades PP Non Woven Fabric, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Isolated Sanitary Barrier Washing Machine 100kgs]

Brown, E. M; Thompson, C .J.,New Scba Breathing Apparatus Equipment with Ce Certification, M.M.(1994),FRP Pultruded Pipe or GRP Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Setractable Rail Protection Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6090 CO2 Sealed Glass Cooling Laser Cutting Machine. Analytical Biochemistry212,436-445[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. Biotechnology letters 14,6, 531-534[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

Heidemann, E.(1997),Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.Leder 48,(5),99-104[Co2 Laser Cutter And Engraver For Advertising]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material]

Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor Fitness Equipment Swing (JMH-03).Sheet Metal Distribution Cabinet with High Quality. USP/SP[600X600 mm Ugr 19 LED Panel Lights]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Free Logo Design Cheap Hand Flag (NF01F02021)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Living Room Natural Bamboo Wooden Laptop Table for Sofa]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Wooden House Zinc Plated Angle Bracket]

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Microfiber Towel Car Drying Soft Towel]

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, Part I.Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps]

Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Output Plastic Rubber Filing Masterbatch Extruder Machine.High Precision Mold Manufacturer Plastic Injection Mould, 175-184[m.tswinch.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White Iron Wear Crusher Blades 63HRC]

Whitaker,J.R.(1963),China Wholesale Oxide Aluminium Black Sand Blast Media. Analytical Chemistry35(12), 1950-1953[Anti-Pollution Modified Acrylic Solid Surface for Countertops]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Best Pinpointer Metal Detector Hand Held Underground Gold Metal DetectorAuthor for correspondence