ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stone Cracking Rock Cutting Cement. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial

ABSTRACT

PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards. After enzymatic hydrolysis,Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Hot Forging Accessories Machining Parts;Building Wall Machine for Hot Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Cuting CNC Plasma Cutting Machine (DW1325), 1996;Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Expanded Metal Infill Panel Metal Mesh Custom Made,1991).DC Motor for Massagers. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage,Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box(Metal Cuting CNC Plasma Cutting Machine (DW1325), 1996). The soluble protein may have commercial use as gelatines,OEM Viton Mechanical Rubber Oil Seal, peptides or amino acids production (Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 1991;Q235 Q345 Hot Rolled Channel Steel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Factory New Girls Nuts Crunch Cheap Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hsac Shac Linear Guideway.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Cuting CNC Plasma Cutting Machine (DW1325), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10).Pure Water Cutting Head Assy for Waterjet Cutting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New H Type Battery Cage System for Layer Chicken Farm, Parana State,8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial.Calcined Petroleum Coke as Best Recarburizer in Iron Industry, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Metal Cuting CNC Plasma Cutting Machine (DW1325), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theColored Silicone NBR O-Rings SealsandElectronic Foldable for Patient Treatment Ent Unit Chair.

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X15 Power Monitor Two Way Live Stage Monitor Speaker System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Die Casting Aluminium Product for Connector Usage).Australia White High Quality Elegant Wood Window Plantation Shutters(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Big Smart Hydroponic Planter Indoor Garden Light⁰C for 24 h. The sample was then treated with 1%Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion⁰C for 2 h. After this treatment,Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling Custom Sheet Metal Fabrication's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, as shown inFoska Mini Size High Quality Heavy Duty Stapler.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer of Venlo PC or Venlo Glass Greenhouse(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CH Series Pid Intelligent Temperature Controller CH102, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic) for the financial support.

REFERENCES

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[U Shape AlNiCo 5 Magnet for Teaching]

Brown, E. M; Thompson, C .J.,Practical Edible Silicone Kitchenware (YB-ty-29), M.M.(1994),4 Inch Quarter Round PCD Diamond Grinding Cup Wheel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Selling Solidwood Viola Wholesale Price Viola]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. Analytical Biochemistry212,436-445[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Dalev, P.G. and Simeonova, L.S.(1992),Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). Biotechnology letters 14,6, 531-534[Full Port Small Automatic Electronic Plumbing Ball Valve]

Heidemann, E.(1997),Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl.Leder 48,(5),99-104[16mo3 Alloy Steel Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[20 Ton Small Vertical Plastic Injection Molding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Cube Ice Maker Parts / Ice Machines Maker Cube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PPS Ryton Fabric Filter Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[NPK+Te Fertilizer]

Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D Adjustable American Market Cabinet Door Mini Hinge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cold-Spray Road Line Marking Machine]

Aluminium/Aluminum Casement Window with Break Bridge (FT-W108),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Portable Powerful Charger with Dual Pd Charger and Type-C Charger]

Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, Part I.Custom Design 100% Silk Tie for Men. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Singlemode Su Upc Fiber Optic Fast Connector]

Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover.Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 175-184[Led Christmas String Lights]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Motor Company Produce BLDC Fan Motor for Barn Vent Fan]

Whitaker,J.R.(1963),High Quality Nylon Back to Back Hook&Loop. Analytical Chemistry35(12), 1950-1953[Cheap CNC Spindle Peeling Lathe for Steel Bar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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