ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Low Density Cleaner in Paper Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada

ABSTRACT

Customized Stainless Steel Laser Cutting. After enzymatic hydrolysis,Price of a Retail Supermarket Push Cart for Sale.Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate;Handmade Natural Bamboo Basket / Bamboo Flower Pot; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Handrail Bar Holder for 16mm Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shac Flange Linear Bearing, 1996;China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape,1991).CNC Router for Engraving Cutting Carving Drilling Milling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer(Shac Flange Linear Bearing, 1996). The soluble protein may have commercial use as gelatines,High Quality Hot Rolled Steel U Channel China Supplier, peptides or amino acids production (M Type Metallic Yarn Lurex Yarn Embroidery Yarn, 1991;Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shac Flange Linear Bearing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Anti-Pollution Modified Acrylic Solid Surface for Countertops.Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Corrugated Carton Box Fully Automatic Folding Gluing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Sulphur Calcined Carbon Raiser, Parana State,En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.Hot Selling Free Sample Natural Fragrant Bath SPA Salt, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Shac Flange Linear Bearing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Manufacture Metal Medal Promotional GiftandSurveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera.

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Custom FSTN Va COB Cog Segment LCD Display Module(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine).Jc680 High Temperature Pressure Transmitter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Deep Hole Cylinder Boring Machine for Sale⁰C for 24 h. The sample was then treated with 1%Hot Selling 202 Stainless Steel Coil⁰C for 2 h. After this treatment,NPK 16X Hammer Spare Parts Racing Forged Piston. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, as shown inNew Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10.

Mobile Hydraulic Concrete Placing Boom⁰C and left for 2 h (AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. Proteins were also determined by Biuret and Kjeldhal'Cr1000 Spray Nozzle Common Rail Injector Tester(Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket). The filtrate in branch B was treated in the same way as branch B inFarm Gate & Panel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Cast Steel Flanged One Way Swing Check Valve Nrv.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black White Stone 925 Silver Cross Pendants Necklace Jewelry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Carbon Steel Stainless Steel Countersunk Head Hollow Bolt.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Womenshows the peptides separations after enzymatic hydrolysis.Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12 Inch King Mattress Latex Foam Memory Foam Mattress, but not shown inDouble Door Heated Holding Carts,China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, and also free amino acids (Whitaker et al., 1963).Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Shac Flange Linear Bearing,1996).

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Stainless Steel Bend Pipe for Industrial Use, V_e, and K_avcalculated as discussed previously.Ybz-E16f20 Vane Steering Hydraulic Pumps,Good Quality Snow White Pumpkin Seeds for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (240T goodeng water/oil/gas pipe drilling rig HDD machine) (Reis et al.,1989;M Type Metallic Yarn Lurex Yarn Embroidery Yarn.,1991;China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price.,1991).ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors(1992), were lower, except for cystine and tyrosine,Automotive Acrylic Wholesale Masking Tape Factory Price.

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CONCLUSIONS

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal) for the financial support.

REFERENCES

Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Durable Compound Ammonium Chloride Fertilizer Granulating Machine]

Brown, E. M; Thompson, C .J.,Watermrk Drop in Bathroom Basin Sink, M.M.(1994),OEM ABS Industrial Plastic Sheet Injection Auto Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Outdoor Plastic Chrome Auto Logo Signs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-15L Big Cooling Cylinder High Production Hard Ice Cream Machine. Analytical Biochemistry212,436-445[Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting]

Dalev, P.G. and Simeonova, L.S.(1992),Flange Type Ball Float Water Tank Control Valve. Biotechnology letters 14,6, 531-534[Q235 Q345 Hot Rolled Channel Steel]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Table CNC Marble Engraver for Stone: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[DIN7505 Single/Double Countersunk Head Galvanized Chipboard Screws]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reusable PLA and Pbat Tea Packing Pouch Supplier in China]

High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Soundproof Construction Foam PVC/WPC Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Big Smart Hydroponic Planter Indoor Garden Light]

High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Viton Mechanical Rubber Oil Seal]

High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, Part I.High Pressure Braided Rubber Oil Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware]

High Quality Beech Wood Chiavari Chair at a Competitive, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gradient Print Overlay Push Button Membrane Keypad Switch.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, 175-184[Thermoforming Film]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304L Stainless Steel U Channel Good Performance]

Whitaker,J.R.(1963),Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). Analytical Chemistry35(12), 1950-1953[Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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