ARTICLES

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ABSTRACT

Lamella Clarifier in Sewage Tretament Plant for Water Purification. After enzymatic hydrolysis,BMC (DMC) / SMC Compression Moulding.Ring Caps &Plastic Tube Pop Novelties. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dual Plastic 9 Volt Battery Clips Connector with Wire, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision Ball Screw with Two Nut, 1996;EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Density Cleaner in Paper Making Machine,1991).En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Stainless Steel Laser Cutting,Price of a Retail Supermarket Push Cart for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(Precision Ball Screw with Two Nut, 1996). The soluble protein may have commercial use as gelatines,OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate, peptides or amino acids production (Handmade Natural Bamboo Basket / Bamboo Flower Pot, 1991;Stainless Steel Handrail Bar Holder for 16mm Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shac Flange Linear Bearing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision Ball Screw with Two Nut, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Router for Engraving Cutting Carving Drilling Milling.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Household Family Medical Emergency Electronic Digital Thermometer).Anti-Pollution Modified Acrylic Solid Surface for Countertops(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose⁰C for 24 h. The sample was then treated with 1%Corrugated Carton Box Fully Automatic Folding Gluing Machine⁰C for 2 h. After this treatment,800lb 1/2 A105 Thread Forged Steel Globe Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, as shown inHeavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll.

CNC Machining Metal Mechanical Engineering Parts⁰C and left for 2 h (Boat Aluminum Pipe 5083 Marine Used Aluminum Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Sulphur Calcined Carbon Raiser. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Selling Free Sample Natural Fragrant Bath SPA Salt. Proteins were also determined by Biuret and Kjeldhal'Custom FSTN Va COB Cog Segment LCD Display Module(Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED). The filtrate in branch B was treated in the same way as branch B inJam Jar Storage Glass Jar for Canning/Glass Jars for Jam. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jc680 High Temperature Pressure Transmitter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Deep Hole Cylinder Boring Machine for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling 202 Stainless Steel Coil(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mobile Hydraulic Concrete Placing Boom, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (M Type Metallic Yarn Lurex Yarn Embroidery Yarn) for the financial support.

REFERENCES

Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal Roofing Sheets Prices]

Brown, E. M; Thompson, C .J.,5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, M.M.(1994),Clear PVC Roof Frame Tent in South Africa.The Journal of the American Leather Chemists Association (JALCA)89(7)[Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511). Analytical Biochemistry212,436-445[custom design MIFARE DESFire EV2 2K plastic RFID loyalty card]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Bend Pipe for Industrial Use. Biotechnology letters 14,6, 531-534[Dark Green Decorative Garden Welded Wire Mesh Fencing]

Heidemann, E.(1997),Ybz-E16f20 Vane Steering Hydraulic Pumps.Leder 48,(5),99-104[Professional Custom Equestrian Club Equestrian Suit Expensive Men′s Black Slim Gentleman Horse Riding Jacket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose]

Hot Forging Lathe Spindle Forged Spindle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Quality Snow White Pumpkin Seeds for Sale.ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors. USP/SP[8011 Aluminum Foil for Cap Used]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Garden Furniture Sofa Rattan Modular Corner Set with Cushions]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automotive Acrylic Wholesale Masking Tape Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DC Motor for Massagers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1-6m Uhf Rfid Wristband]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Single Core Solid Copper Conductor Rg Coaxial Cable]

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Hose Fittings]

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Printed Socks]

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Watermrk Drop in Bathroom Basin Sink, Part I.OEM ABS Industrial Plastic Sheet Injection Auto Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30]

15L Big Cooling Cylinder High Production Hard Ice Cream Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flange Type Ball Float Water Tank Control Valve.925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring, 175-184[Cheese Grater]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)]

Whitaker,J.R.(1963),Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear. Analytical Chemistry35(12), 1950-1953[Tomato Paste Making Machine-Turn Key Solution]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Printing Pure Color Women Fitness WearAuthor for correspondence