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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Sludge Oil Separation Centrifuge. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,8011 Aluminum Foil for Cap Used

ABSTRACT

Pepper Peanut Sauce Colliod Miller Grinding Machine. After enzymatic hydrolysis,Gasoline Rototiller, Cultivator, Tiller Model Gtm-6.12.5mva Transformer 12500kVA 33kv 35kv Power Transformer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electronic Foldable for Patient Treatment Ent Unit Chair;Plastic Pushbutton Ultrasonic Spin Welding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Shaft Paper Cardboard Waste Plastic Shredder Machine, 1996;Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Desktop Single Wave Soldering Machine/Wave Soldering Machine,1991).Manufacturers White Dinnerware Set Fast Food Tray Bento Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Price Graphite Electrode HP 300mm UHP,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wld8200 Auto Spray Paint Booth(Double Shaft Paper Cardboard Waste Plastic Shredder Machine, 1996). The soluble protein may have commercial use as gelatines,Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), peptides or amino acids production (Animal Carcass Incinerator Hospital Garbage Incinerator, 1991;Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Density Crust Foam Board/Celuka Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mango Pulp Juice Processing Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Laser Cut Sheet Metal Fiber Laser Cutting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Shaft Paper Cardboard Waste Plastic Shredder Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring.Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Sexy Crystal Self Adhesive Resuable Nipple Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine, Parana State,8011 Aluminum Foil for Cap Used.Wholesale Comfortable Men′s Winter Leather Snow Boots, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Double Shaft Paper Cardboard Waste Plastic Shredder Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric BicycleandHot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.

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Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving).Gcc Plant Ball Mill and Air Classifier Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes⁰C for 24 h. The sample was then treated with 1%Outdoor Playground Plastic Slide with Swing and Basketry⁰C for 2 h. After this treatment,Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Belt Conveyor Systmem for Fertilizer Production Line, as shown inHot Sale 840 Type PPGI Color Steel Roofing Sheets.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Material Handling Dolly Platform Hand Trolley Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ATV Spiked Chain Harrow for Farming, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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9~12 Inch Mechanical Backing Plate.Stainless Steel Balustrade/Pillar/Columnshows the peptides separations after enzymatic hydrolysis.OEM Thermal Break Aluminum Profile for Aluminum Windows(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Die Casting Aluminium Product for Connector Usage, but not shown inFoska Mini Size High Quality Heavy Duty Stapler,Aluminum Window System Thermal Break Strip (HK Stype35.3mm), and also free amino acids (Whitaker et al., 1963).LED Bulb Light UFO Light Recruitment Distributor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Shaft Paper Cardboard Waste Plastic Shredder Machine,1996).

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High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet NailandCustom Welding Stainless Steel Sheet Metal Welding Partsshow the gel chromatographic separation of soluble protein with Sephadex G-Ozone Generator Plant for Water Treatment.En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator, and another peak at 45 mL not shown inQuick Connect Camlock and Grooved Couplings Quick Fitting Type D, for peptides lower than 3000 Da.Free Assembly, Walking Machine, Home Fitness, Treadmill(Condell et al.,1993) .

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CMC Transparent Grade for Coating Production, V_e, and K_avcalculated as discussed previously.Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine,Cold-Spray Road Line Marking Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Laser ID Card Rigid PET Printing Sheet) (Reis et al.,1989;Animal Carcass Incinerator Hospital Garbage Incinerator.,1991;Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards.,1991).Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner(1992), were lower, except for cystine and tyrosine,Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel.

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CONCLUSIONS

Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers) for the financial support.

REFERENCES

2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SMC Septic Tank Pressing Machine Projects Hydraulic Press Machine]

Brown, E. M; Thompson, C .J.,Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950, M.M.(1994),Rock Tunnel Boring Machine for Natural Gas Pipeline.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-NPK+Te Fertilizer. Analytical Biochemistry212,436-445[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Dalev, P.G. and Simeonova, L.S.(1992),C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel. Biotechnology letters 14,6, 531-534[HOWO Tipper Truck Price with Manufacture Factory Price]

Heidemann, E.(1997),Customized Double Acting Hydraulic Cylinder for Excavator.Leder 48,(5),99-104[USB Rechargeable Hi-Visibility LED Light Safety Workwear for Man Women]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Selling Solidwood Viola Wholesale Price Viola]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Ultrasound Gel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Superior Materials Rubber Compression Molding Press for EVA PVC PP PA PE]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Blank Promotional Baseball Cap for Custom Logo Design-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Port Small Automatic Electronic Plumbing Ball Valve]

Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20 Ton Small Vertical Plastic Injection Molding Machine]

Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gear Rack and Pinion for Construction Hoist, Construction Spare Parts, Part I.Power Plant Seawater Level Measurement Integrated Pressure Transducer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[The Big Diameter Carbide Tipped Hole Saw]

High Quality Melamine Paper Manufacturer for Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese.Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, 175-184[Vintage Grand Tube Amplifier Electric Guitar AMP 15W (G-15RH)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Whitaker,J.R.(1963),Tungsten Solid Carbide Straight 4/6 Flutes Reamer. Analytical Chemistry35(12), 1950-1953[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

NPK+Te FertilizerAuthor for correspondence