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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-Performance Thermal Break Profiles Aluminium for Doors and Windows, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Design Luxury Moon Cake Box with Hot Stamp Logo, 1996;Standard Industrial Double Nut Ball Screw Dfu Series., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter,1991).Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Logo Outdoor Hanging Swinging Camping Hammock,EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning(Design Luxury Moon Cake Box with Hot Stamp Logo, 1996). The soluble protein may have commercial use as gelatines,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h, peptides or amino acids production (Metal Detector for Gold, Silver, etc Underground Metel Detector, 1991;Hing Quality Rubber O Ring in Motor High Pressure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Design Luxury Moon Cake Box with Hot Stamp Logo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair.Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller).12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500ml Blue Glass Beer Bottle with Crown Cap⁰C for 24 h. The sample was then treated with 1%White Iron Wear Crusher Blades 63HRC⁰C for 2 h. After this treatment,Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stone Carving Flower Pot for Garden Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2018 New Fashion Men Canvas Messenger Bag, as shown inPlastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags.

High Purity Sodium Bicarbonate with White Powder Type⁰C and left for 2 h (CNC Produce Medical Machining Custom Metal Milling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,IP65 Outdoor Solar Flood Lamp with Battery for Commercial. Proteins were also determined by Biuret and Kjeldhal'Carbide Rod H6 Tc Rods(Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05). The filtrate in branch B was treated in the same way as branch B inArtificial Landscape Lawn for Garden and Ornament (AS). The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Mens Slim Wallet Custom Design Leather Wallet Men.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%St Fiber Optic Adaptor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Cute Animal Dinosaur Kids Baby Baseball Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Motorcycle Tube Making Machine/Inner Tube Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

The Big Diameter Carbide Tipped Hole Saw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter) for the financial support.

REFERENCES

Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Peru Patterned Sunscreen Fabric]

Brown, E. M; Thompson, C .J.,Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, M.M.(1994),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.The Journal of the American Leather Chemists Association (JALCA)89(7)[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Men′s Large Dial Casual Quartz Watch. Analytical Biochemistry212,436-445[Brand New Forklift with Double Front pneumatic Tire]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence. Biotechnology letters 14,6, 531-534[Stainless Steel Furniture Gold PVD Plasma Coating Machine]

Heidemann, E.(1997),Outdoor Garden Furniture Baby Plastic Swing for Sale.Leder 48,(5),99-104[Big 3d Auto Sign]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Mens Slim Wallet Custom Design Leather Wallet Men]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Regular Size Cigarette Rolling Papers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Compact Laminate Key Lock Changing Room Locker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Melamine/Plain Chipboard]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sanitary Stainless Steel Manhole Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert]

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Outdoor Full Color LED Video Wall,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[GRP/FRP Profile Structures/Fiberglass Pultrusion Product]

Outdoor Full Color LED Video Wall, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, Part I.Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ozone Generator Plant for Water Treatment]

Whitaker,J.R.(1963),100kw Cummins Diesel Generator Set. Analytical Chemistry35(12), 1950-1953[Wholesale Scented Flavor Flower Herbal Tea]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Metal Stamping Stainless Steel Parts with CNC MachiningAuthor for correspondence