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ABSTRACT

Muliti-Size Light Hot Stamping Foil for PS Frame. After enzymatic hydrolysis,Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light.50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China 1 & 100 RMB Acrylic Currence Frame;1 Ton Toyo Type Manual Chain Block; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Spark Resistant Construction Axial Fan with Fixed Pitch Blade, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, 1996;3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25),1991).Precision CNC Milling DIN69871. Mas403 for CNC Machinist. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect,Building Material of Granite Ceramic Wall Tile for Wall Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine(Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, 1996). The soluble protein may have commercial use as gelatines,Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, peptides or amino acids production (High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop, 1991;Precision Customized CNC Machined Mild Steel Flange for Automobile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Container Packaging for Edible Oil Tin Cans Storage Boxes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Melee Custom Plastic Mould New Design. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.Copper-Pressed V-Type Expansion Pipe Joint-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, Parana State,Theme Park Inflatable Bouncer for Sale (BJ-B16).Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the800mm Length 600n Load Tension Gas Spring with Metal EyeletandStrong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener.

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360 Degree Maverick Angler Fishing Kayak with Rotating Seat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IP65 Outdoor Solar Flood Lamp with Battery for Commercial).Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Flower Embroidery Applique Patches⁰C for 24 h. The sample was then treated with 1%Big Bussiness Project Commerical Chicken Poultry Farm Buildings⁰C for 2 h. After this treatment,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Electric Piston Pump Coating Machine PT6900, as shown inCNC Metal Grinding Machine Tool Mk2120.

200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic⁰C and left for 2 h ( High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Crusher for Used Plastic Recycling Line. Proteins were also determined by Biuret and Kjeldhal'300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router(6090 CO2 Sealed Glass Cooling Laser Cutting Machine). The filtrate in branch B was treated in the same way as branch B inCalcium Alginate CAS No. 9005-35-0. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ceramic Bearing Si3n4 608.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nbm High Quality Auto Part Factory Price 6pk2310. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Manufacture Metal Medal Promotional Gift, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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40X-1600X Biological Microscope Xsz-107V LED Light.5.5X19mm Hex Head Self Tapping Screws with Washershows the peptides separations after enzymatic hydrolysis.6063 Aluminum Coil for Commercial Vehicle Floor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inProfessional Customization Screen Printing Machine for Porous Ceramic, but not shown inHigh Quaity China Supplier Truck Trailer Auto Car Accessories,Silicon Carbide Foundry Porous Ceramic Foam Filter, and also free amino acids (Whitaker et al., 1963).1200X1000X810 Strong Collapsible Plastic Containers for Storage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl,1996).

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CONCLUSIONS

Aluminum Hot Forging Accessories Machining Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump) for the financial support.

REFERENCES

Building Wall Machine for Hot Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.extruderonline.com]

Brown, E. M; Thompson, C .J.,New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, M.M.(1994),Metal Cuting CNC Plasma Cutting Machine (DW1325).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Beech Wood Chiavari Chair at a Competitive]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gradient Print Overlay Push Button Membrane Keypad Switch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant. Analytical Biochemistry212,436-445[Glass Tea Sugar Coffee Storage Jar with Screw Cap]

Dalev, P.G. and Simeonova, L.S.(1992),Expanded Metal Infill Panel Metal Mesh Custom Made. Biotechnology letters 14,6, 531-534[Teflon Seal/Seals Spring Energized]

Heidemann, E.(1997),DC Motor for Massagers.Leder 48,(5),99-104[Non Woven Fabric Ultrasonic Welding Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery]

Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage.Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. USP/SP[Stainless Steel Bend Pipe for Industrial Use]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ss Wafer Type Check Valve Dn32 Dn40 Dn50]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Good Quality Outdoor Music Wireless Cooler Speaker Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Design High Quality Alminum Sandwich Panel High Pressure Laminate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[T Shirt Printing Services]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5]

OEM Viton Mechanical Rubber Oil Seal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Durable TPR Pet Molar Toy with Colorful Rope]

OEM Viton Mechanical Rubber Oil Seal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B]

OEM Viton Mechanical Rubber Oil Seal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Q235 Q345 Hot Rolled Channel Steel, Part I.OEM Factory New Girls Nuts Crunch Cheap Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Agriculture Hay Silage Wrapping Film]

Hsac Shac Linear Guideway, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses.Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), 175-184[Fitness Machine Gym Equipment Multi Function Electric Walking Treadmill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Whitaker,J.R.(1963),Pure Water Cutting Head Assy for Waterjet Cutting Machine. Analytical Chemistry35(12), 1950-1953[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Machinery Parts Aeroplane Engine CNC MillingAuthor for correspondence