ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine

ABSTRACT

CMP No Nc Metal Switch Black 25mm Illuminated Push Button. After enzymatic hydrolysis,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Felt Disc Polishing Sanding Disc with Wool Felt Diam;13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, 1996;240T goodeng water/oil/gas pipe drilling rig HDD machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas,1991).Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen,Colorful Braided Fabric Cable Round,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Universal Wholesale Hot Sale Custom Blank Keys(100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, 1996). The soluble protein may have commercial use as gelatines,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, peptides or amino acids production (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1991;Waw 8 Ton Logistics Light Truck for Transportation, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Forging Lathe Spindle Forged Spindle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Chic Straw Braid Floppy Hat with Print Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lamella Clarifier in Sewage Tretament Plant for Water Purification.BMC (DMC) / SMC Compression Moulding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ring Caps &Plastic Tube Pop Novelties(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, Parana State,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine.Low Density Cleaner in Paper Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDeep Hole Cylinder Boring Machine for SaleandCustom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.

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En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box).Customized Stainless Steel Laser Cutting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Price of a Retail Supermarket Push Cart for Sale⁰C for 24 h. The sample was then treated with 1%Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable⁰C for 2 h. After this treatment,OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Handmade Natural Bamboo Basket / Bamboo Flower Pot's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Handrail Bar Holder for 16mm Pipe, as shown in500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet.

Shac Flange Linear Bearing⁰C and left for 2 h (Aws E7018 Low Carbon Steel Welding Electrode). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. Proteins were also determined by Biuret and Kjeldhal'CNC Router for Engraving Cutting Carving Drilling Milling(Newest Men Watches Popular Products Mens Custom Wood Watch). The filtrate in branch B was treated in the same way as branch B inCustom Logo Decorative Gift Bow Stain Ribbon for Christmas. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEmpty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.M Type Metallic Yarn Lurex Yarn Embroidery Yarn⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Bladesand500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. Enzymatic (YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable)Anti-Pollution Modified Acrylic Solid Surface for Countertops(sulfuric acid)Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Corrugated Carton Box Fully Automatic Folding Gluing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Corrugated Carton Box Fully Automatic Folding Gluing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

800lb 1/2 A105 Thread Forged Steel Globe Valve.Life Size 170cm Silicone Sex Dolls Real Love Doll for Maleshows the peptides separations after enzymatic hydrolysis.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Design 100% Silk Tie for Men, but not shown inAccurate Measuring Rulers 3D Lenticular Plastic Rulers,China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, and also free amino acids (Whitaker et al., 1963).CNC Machining Metal Mechanical Engineering Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth,1996).

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2PC Flanged&ThreadedandGalvanized Steel Overhead Highway Signage Platforms Grating Trench Covershow the gel chromatographic separation of soluble protein with Sephadex G-Low Sulphur Calcined Carbon Raiser.Hot Selling Free Sample Natural Fragrant Bath SPA Salt, and another peak at 45 mL not shown inPatchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, for peptides lower than 3000 Da.Custom FSTN Va COB Cog Segment LCD Display Module(Condell et al.,1993) .

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Jc680 High Temperature Pressure Transmitter, V_e, and K_avcalculated as discussed previously.Deep Hole Cylinder Boring Machine for Sale,Hot Selling 202 Stainless Steel Coil. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper) (Reis et al.,1989;Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30.,1991;240T goodeng water/oil/gas pipe drilling rig HDD machine.,1991).NPK 16X Hammer Spare Parts Racing Forged Piston(1992), were lower, except for cystine and tyrosine,2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve.

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CONCLUSIONS

Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dual Plastic 9 Volt Battery Clips Connector with Wire) for the financial support.

REFERENCES

Mobile Hydraulic Concrete Placing Boom, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.sajoo-switch.com]

Brown, E. M; Thompson, C .J.,Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, M.M.(1994),Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll.The Journal of the American Leather Chemists Association (JALCA)89(7)[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cr1000 Spray Nozzle Common Rail Injector Tester. Analytical Biochemistry212,436-445[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

Dalev, P.G. and Simeonova, L.S.(1992),Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. Biotechnology letters 14,6, 531-534[Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material]

Heidemann, E.(1997),Black White Stone 925 Silver Cross Pendants Necklace Jewelry.Leder 48,(5),99-104[Waterproof WPC Celuca Foam Board WPC Wall Panel WPC Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[600X600 mm Ugr 19 LED Panel Lights]

Tefude Feeding and Packaging Line for Food with Tray, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People.Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. USP/SP[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Free Logo Design Cheap Hand Flag (NF01F02021)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Wooden House Zinc Plated Angle Bracket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Corner Angle Joint Aluminum Die Casting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Microfiber Towel Car Drying Soft Towel]

Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps]

Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, Part I.China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White Iron Wear Crusher Blades 63HRC]

Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator, 175-184[Submersible Fuel Pressure Sensor for Monitoring Fuel Level of Generators Tanks]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti-Pollution Modified Acrylic Solid Surface for Countertops]

Whitaker,J.R.(1963),Carbon Steel Stainless Steel Countersunk Head Hollow Bolt. Analytical Chemistry35(12), 1950-1953[Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Gcc Plant Ball Mill and Air Classifier Production LineAuthor for correspondence