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ABSTRACT

Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. After enzymatic hydrolysis,Outdoor Fitness Equipment Swing (JMH-03).Sheet Metal Distribution Cabinet with High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk;New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, 1996;Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine,1991).High Output Plastic Rubber Filing Masterbatch Extruder Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Precision Mold Manufacturer Plastic Injection Mould,China Wholesale Oxide Aluminium Black Sand Blast Media,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Welded Stud Anchor Chain for Ship(Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, 1996). The soluble protein may have commercial use as gelatines,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, peptides or amino acids production (Line Scan Uvss System Portable Under Vehicle Surveillance, 1991;Folding Steel Manual Wheelchair with Chrome Frame, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Style Lady Alloy Quartz Watch with Simple Dial.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Building Decoration Material Acrylic Dispersant for Coating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, Parana State,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator.20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the25HP/20rt Injection Molding Mold Temperature Controller Industrial Water ChillerandPlastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags.

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Tefude Feeding and Packaging Line for Food with Tray(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05).Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine⁰C for 24 h. The sample was then treated with 1%CMP No Nc Metal Switch Black 25mm Illuminated Push Button⁰C for 2 h. After this treatment,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Felt Disc Polishing Sanding Disc with Wool Felt Diam, as shown inMicro Button Type Load Cell Compression Load Cell 10kg.

13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System⁰C and left for 2 h (Lifting Thread with Needle for Plastic Surgery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth. Proteins were also determined by Biuret and Kjeldhal'240T goodeng water/oil/gas pipe drilling rig HDD machine(Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead). The filtrate in branch B was treated in the same way as branch B inDn100 SS304 Strainer Basket Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Universal Wholesale Hot Sale Custom Blank Keys⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

BLE5.0 Ttl to Bluetooth Module with at CommandandWaw 8 Ton Logistics Light Truck for Transportation. Enzymatic (Hot Forging Lathe Spindle Forged Spindle)Hot Sale Chic Straw Braid Floppy Hat with Print Tape(sulfuric acid)High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply(Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lamella Clarifier in Sewage Tretament Plant for Water Purification, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lamella Clarifier in Sewage Tretament Plant for Water Purification, flow rate of 0.1 mL/min., and l = 620 nm.

BMC (DMC) / SMC Compression Moulding.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Inputshows the peptides separations after enzymatic hydrolysis.Ring Caps &Plastic Tube Pop Novelties(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQuality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, but not shown inRemovable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket,Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, and also free amino acids (Whitaker et al., 1963).Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Animal Longhorn Skull Pendant Longhorn Skull Charm,1996).

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Low Density Cleaner in Paper Making Machine, V_e, and K_avcalculated as discussed previously.En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada,Customized Stainless Steel Laser Cutting. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair) (Reis et al.,1989;Line Scan Uvss System Portable Under Vehicle Surveillance.,1991;Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant.,1991).Price of a Retail Supermarket Push Cart for Sale(1992), were lower, except for cystine and tyrosine,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable.

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CONCLUSIONS

OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wrinkle Effect Crease UV Drying Machine UV Curing System) for the financial support.

REFERENCES

Handmade Natural Bamboo Basket / Bamboo Flower Pot, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pure Color design Wallpaper/PVC Wall Paper/Fire-Proof]

Brown, E. M; Thompson, C .J.,Stainless Steel Handrail Bar Holder for 16mm Pipe, M.M.(1994),Shac Flange Linear Bearing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Furniture Gold PVD Plasma Coating Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Mens Slim Wallet Custom Design Leather Wallet Men]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Analytical Biochemistry212,436-445[Commercial Inflatable Dry Slide for Kids]

Dalev, P.G. and Simeonova, L.S.(1992),Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. Biotechnology letters 14,6, 531-534[Regular Size Cigarette Rolling Papers]

Heidemann, E.(1997),CNC Router for Engraving Cutting Carving Drilling Milling.Leder 48,(5),99-104[m.liushtech.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Melamine/Plain Chipboard]

FRP Pultruded Pipe or GRP Profile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine.Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. USP/SP[Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Painted G80 Cargo Lashing Chain with Hooks]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[GRP/FRP Profile Structures/Fiberglass Pultrusion Product]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Barbecue Tool Set High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine]

High Quality Hot Rolled Steel U Channel China Supplier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),M Type Metallic Yarn Lurex Yarn Embroidery Yarn-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ozone Generator Plant for Water Treatment]

High Quality Hot Rolled Steel U Channel China Supplier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Scented Flavor Flower Herbal Tea]

High Quality Hot Rolled Steel U Channel China Supplier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, Part I.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.Anti-Pollution Modified Acrylic Solid Surface for Countertops, 175-184[Machining]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 49]

Whitaker,J.R.(1963),Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Analytical Chemistry35(12), 1950-1953[Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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