ARTICLES

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ABSTRACT

High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. After enzymatic hydrolysis,Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades.Factory Supply Straight Ball Joint Bearing 02b04. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wrinkle Effect Crease UV Drying Machine UV Curing System;Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1996;Tefude Feeding and Packaging Line for Food with Tray., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts,1991).Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CMP No Nc Metal Switch Black 25mm Illuminated Push Button,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil(20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1996). The soluble protein may have commercial use as gelatines,Felt Disc Polishing Sanding Disc with Wool Felt Diam, peptides or amino acids production (13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, 1991;Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.240T goodeng water/oil/gas pipe drilling rig HDD machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength.Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Colorful Braided Fabric Cable Round(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Forging Lathe Spindle Forged Spindle, Parana State,Building Decoration Material Acrylic Dispersant for Coating.Hot Sale Chic Straw Braid Floppy Hat with Print Tape, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial UseandHigh Quality Crusher for Used Plastic Recycling Line.

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High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine).Lamella Clarifier in Sewage Tretament Plant for Water Purification(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%BMC (DMC) / SMC Compression Moulding⁰C for 24 h. The sample was then treated with 1%Ring Caps &Plastic Tube Pop Novelties⁰C for 2 h. After this treatment,Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dual Plastic 9 Volt Battery Clips Connector with Wire, as shown inLow Sulphur Calcined Carbon Raiser.

Precision Ball Screw with Two Nut⁰C and left for 2 h (12V 24V 220V Digital Temperature Controller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Density Cleaner in Paper Making Machine. Proteins were also determined by Biuret and Kjeldhal'En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada((JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine). The filtrate in branch B was treated in the same way as branch B inPower Recliner Home Theater Seating. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Stainless Steel Laser Cutting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Price of a Retail Supermarket Push Cart for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Handmade Natural Bamboo Basket / Bamboo Flower Pot⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Handrail Bar Holder for 16mm Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shac Flange Linear Bearing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cattle Corpse Cremation Burning StoveandChina Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Enzymatic (Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape)CNC Router for Engraving Cutting Carving Drilling Milling(sulfuric acid)Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine(20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30) for the financial support.

REFERENCES

CNC Machining Metal Mechanical Engineering Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.gsrfid.com]

Brown, E. M; Thompson, C .J.,Low Sulphur Calcined Carbon Raiser, M.M.(1994),Hot Selling Free Sample Natural Fragrant Bath SPA Salt.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quaity China Supplier Truck Trailer Auto Car Accessories]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom FSTN Va COB Cog Segment LCD Display Module. Analytical Biochemistry212,436-445[Professional Exporter of Wheat Flour Mill Plant (80t)]

Dalev, P.G. and Simeonova, L.S.(1992),Jc680 High Temperature Pressure Transmitter. Biotechnology letters 14,6, 531-534[Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)]

Heidemann, E.(1997),Deep Hole Cylinder Boring Machine for Sale.Leder 48,(5),99-104[Custom PP Frosting Clear Plastic Storage Boxes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wood Chips Rotary Dryer Sawdust Pellet Drying Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fly Ash Brick Machine Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence]

Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mobile Hydraulic Concrete Placing Boom-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Beech Wood Chiavari Chair at a Competitive]

Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gradient Print Overlay Push Button Membrane Keypad Switch]

Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, Part I.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glass Tea Sugar Coffee Storage Jar with Screw Cap]

Cr1000 Spray Nozzle Common Rail Injector Tester, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Cast Steel Flanged One Way Swing Check Valve Nrv.Black White Stone 925 Silver Cross Pendants Necklace Jewelry, 175-184[Essential Oil Glass Round Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Teflon Seal/Seals Spring Energized]

Whitaker,J.R.(1963),Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. Analytical Chemistry35(12), 1950-1953[Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Bend Pipe for Industrial UseAuthor for correspondence