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ABSTRACT

Competitive Price Sodium Gluconate as Concrete Admixture. After enzymatic hydrolysis,Precision Investment Casting Stainless Steel 304 Wire Rope Hook.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Die Casting Aluminium Product for Connector Usage;Stainless Steel Balustrade/Pillar/Column; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PP/Pet Woven Geotextile for Road Construction, 1996;Lamella Clarifier in Industrial Wastewater Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering,1991).Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights,Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts(PP/Pet Woven Geotextile for Road Construction, 1996). The soluble protein may have commercial use as gelatines,IS Type Cast Iron Chemical Industrial Pump, peptides or amino acids production (Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine, 1991;Custom Grooved Quartz Plate Deep Processing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale CNC Router Machine for Wood Cutting (1325)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50g Body Luxury King Crown Cosmetic Containers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PP/Pet Woven Geotextile for Road Construction, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Paper Egg Box Forming Molding Production Line/ Egg Tray Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, Parana State,Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof.Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PP/Pet Woven Geotextile for Road Construction, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTransparent 43/410 Soap Dispenser Hand Wash Pump Foam PumpandPP Coated Pipe Machine Extruder Machine.

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12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Autoclavable Surgical Thoracoscopy Trocar).Hydraulic Electric Stationary Car Platform Scissor Elevator Lift(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building⁰C for 24 h. The sample was then treated with 1%Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal⁰C for 2 h. After this treatment,Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), as shown inHigh Temperature Side Opening AAC Autoclave.

Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale⁰C and left for 2 h (Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quaity China Supplier Truck Trailer Auto Car Accessories. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. Proteins were also determined by Biuret and Kjeldhal'Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light(Waterjet 87K Intensifier Part Check Valve Body 020077-1). The filtrate in branch B was treated in the same way as branch B inLED Display Ammter for Switchgear Cabinets Ammeter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Steel Investment Casting High Hardness Agricultural Machinery Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Minimalist Slim Real Leather Front Pocket Card Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNatural Cleaning Konjak Sponge(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Biodegradable Disposible Straw Pulp Food Box Ppp-1007⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag.Small Table CNC Marble Engraver for Stoneshows the peptides separations after enzymatic hydrolysis.Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBrown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, but not shown inAd-L13 Black Perfume Plastics Vial Bottle 10ml,500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet, and also free amino acids (Whitaker et al., 1963).12 Inch King Mattress Latex Foam Memory Foam Mattress. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PP/Pet Woven Geotextile for Road Construction,1996).

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Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable, V_e, and K_avcalculated as discussed previously.Longyi Crimping Types Cable Terminal Lugs,Rigid PVC Foam Board for FRP Marine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip) (Reis et al.,1989;Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.,1991;Lamella Clarifier in Industrial Wastewater Treatment.,1991).Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display(1992), were lower, except for cystine and tyrosine,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45).

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CONCLUSIONS

Stainless Steel Furniture Gold PVD Plasma Coating Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Metal Grinding Machine Tool Mk2120) for the financial support.

REFERENCES

Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Celing Drains]

Brown, E. M; Thompson, C .J.,Steel Pipe Rust Removal Shot Blasting Cleaning Machine, M.M.(1994),Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace. Analytical Biochemistry212,436-445[Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Bread Crumb Processing Line and Making Extruder Machine. Biotechnology letters 14,6, 531-534[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

Heidemann, E.(1997),Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder.Leder 48,(5),99-104[Membrane Keyboard Switch with Clear Window]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair]

High Quality Women′s Suit in Reasonable Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring.300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve. USP/SP[ etc Underground Metel Detector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Injection Molding Companies China for Tablet PC Case: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Laser Cut Sheet Metal Fiber Laser Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Round High Quality Fr4 PCB Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape]

27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Solid Tungsten Carbide Rods-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Double Shaft Paper Cardboard Waste Plastic Shredder Machine]

27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Building Material of Granite Ceramic Wall Tile for Wall Decoration]

27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, Part I.Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Flange Housing Ucf310-115 for Electric Hand Tools]

High Tensisle Strength Tissue Paper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tractor Mounted Beach Cleaning Equipment Large Size.100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, 175-184[8079 Household Foil for Food Packaging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rock Tunnel Boring Machine for Natural Gas Pipeline]

Whitaker,J.R.(1963),Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts. Analytical Chemistry35(12), 1950-1953[High Purity Sodium Bicarbonate with White Powder Type]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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