ARTICLES

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ABSTRACT

Chromium Oxide Green 99% for Construction and Plastics. After enzymatic hydrolysis,Zs Round Vibrating Sieve.Stainless Steel Tactile Paving Blind Road Stud. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Roller Hearth Heating Furnace for Gas Cylindrer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, 1996;Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hotel Card Door Lock Access Control Safe Door Lock,1991).Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel DIN7981 Pan Head Self Tapping Screw,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax(custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, 1996). The soluble protein may have commercial use as gelatines,Dark Green Decorative Garden Welded Wire Mesh Fencing, peptides or amino acids production (Csm DIY Microscope for Kids/Toy Microscope, 1991;Oak Veneer Laminated Plywood for Decoration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed HDMI Cable Wholesale HDMI with Ethernet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine.High Quality Women′s Suit in Reasonable Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet Short Fiber Non Woven Geotextile for Landfill Filtration(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Teflon Seal/Seals Spring Energized).PP/Pet Woven Geotextile for Road Construction(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lamella Clarifier in Industrial Wastewater Treatment⁰C for 24 h. The sample was then treated with 1%FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering⁰C for 2 h. After this treatment,Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, as shown inMetal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av50g Body Luxury King Crown Cosmetic Containers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5shows the peptides separations after enzymatic hydrolysis.Hydraulic Electric Stationary Car Platform Scissor Elevator Lift(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDurable TPR Pet Molar Toy with Colorful Rope, but not shown in12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B,Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, and also free amino acids (Whitaker et al., 1963).Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (custom design MIFARE DESFire EV2 2K plastic RFID loyalty card,1996).

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CONCLUSIONS

Minimalist Slim Real Leather Front Pocket Card Wallet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Investment Casting Stainless Steel 304 Wire Rope Hook) for the financial support.

REFERENCES

Natural Cleaning Konjak Sponge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lift Type Unidirectional Thin Check Valve]

Brown, E. M; Thompson, C .J.,Indoor Load Break Switch Isolating Switch, M.M.(1994),Biodegradable Disposible Straw Pulp Food Box Ppp-1007.The Journal of the American Leather Chemists Association (JALCA)89(7)[Buddhist Mood Picture Wallpaper Murals]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Garden Furniture Baby Plastic Swing for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card. Analytical Biochemistry212,436-445[Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment. Biotechnology letters 14,6, 531-534[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Heidemann, E.(1997),NBR Latex Adhesive for Glove.Leder 48,(5),99-104[Commercial Glass Door Lock Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Feeding Customized Multi Layers Shirts Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electromagnetic Pulse Valve with DMF-Z-20 / 25 Series]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality]

Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable]

Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Fitness Equipment Swing (JMH-03)]

Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer, Part I.500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4K Giant Whiteboard Interactive Panel for University Whiteboard]

12 Inch King Mattress Latex Foam Memory Foam Mattress, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook.HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, 175-184[Tungsten Bar Tig Welding]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting]

Whitaker,J.R.(1963),Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt. Analytical Chemistry35(12), 1950-1953[100kw Cummins Diesel Generator Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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