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ABSTRACT

100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine. After enzymatic hydrolysis,New Styles Fashion Erotic Women Underwear Sexy Lingerie.High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fully Automatic Water Bottle Lids Labeling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels, 1996;New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs,1991).Fused Silica Quartz Lump 5-50mm for Refractory Materials. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cold Drawn D2 H13 Alloy Steel Turning Roll,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks(2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels, 1996). The soluble protein may have commercial use as gelatines,High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved, peptides or amino acids production (Colorful 100LED Copper String Light Outdoor LED, 1991;H365 Emas Chainsaw Spares Cylinder Kits, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.A4 Diploma Covers Wholesale with Silver Foil.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap.120L 240L Oil Universal Chemical Spil Absorbent Kits-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Newest Men Watches Popular Products Mens Custom Wood Watch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Wholesale Melamine Garment Shop Furniture Design for Store).Accurate Measuring Rulers 3D Lenticular Plastic Rulers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Reusable PLA and Pbat Tea Packing Pouch Supplier in China⁰C for 24 h. The sample was then treated with 1%Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry⁰C for 2 h. After this treatment,Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial P-Toluene Sulfonic Acid for 93%-99% Purity's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, as shown inLtmg Classic Product 6 Ton Wheel Front Loader for Sale.

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair⁰C and left for 2 h (China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Purity99.95% Cobalt Oxide. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wood Activated Carbon for Decolorizing in Food Industry with ASTM. Proteins were also determined by Biuret and Kjeldhal'Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car(275ml Frosted Clear Glass Drinking Bottle for Juice). The filtrate in branch B was treated in the same way as branch B inOEM Men′s Silicone Ring for Promotion Gift. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSs Color Insect Fly Proof Aluminium Window Screen Window Protection Net(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6000 Series Anodizing Extrusion Aluminum Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine.Hot Forging Lathe Spindle Forged Spindleshows the peptides separations after enzymatic hydrolysis.Hot Selling Solidwood Viola Wholesale Price Viola(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHouseholding High Precision Electrical Plastic Injection Parts/Mould, but not shown inWholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant,Building Material Sound Heat Insulation Rock Wool Sandwich Panel, and also free amino acids (Whitaker et al., 1963).Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels,1996).

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Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, V_e, and K_avcalculated as discussed previously.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts,Household Family Medical Emergency Electronic Digital Thermometer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Insulation Top Hung Aluminum Awning Window with Mosquito Net) (Reis et al.,1989;Colorful 100LED Copper String Light Outdoor LED.,1991;New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera.,1991).Die Cast Iron Worm Gear Speed Reduce Gearbox(1992), were lower, except for cystine and tyrosine,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting.

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CONCLUSIONS

High Quality Couple Watches Steel Material Gift Watch OEM in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush) for the financial support.

REFERENCES

Glass Tea Sugar Coffee Storage Jar with Screw Cap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electroplated Polishing Disc]

Brown, E. M; Thompson, C .J.,Circular DC Electro Lifting Magnet for Lifting Scraps, M.M.(1994),Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Particle Board Manufacturer, Melamine/Plain Chipboard. Analytical Biochemistry212,436-445[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. Biotechnology letters 14,6, 531-534[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Heidemann, E.(1997),Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).Leder 48,(5),99-104[Cast Grinding Ball With Any Model]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tk 4100]

Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. USP/SP[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Grooved Quartz Plate Deep Processing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-120ml Glass Perfume Bottles for Designer Perfume: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ISO Ce TUV Quality Assured Tree Debarker Wood Peeling Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Commercial Double Lane Inflatable Carnival Bungee Run Game]

Agriculture Hay Silage Wrapping Film, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Agriculture Hay Silage Wrapping Film,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Agriculture Hay Silage Wrapping Film, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, Part I.16oz Insulated Cardboad Cup Sleeve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W]

Large Outdoor Simple Gazebo Beer Festival Marquee Tent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),HOWO Tipper Truck Price with Manufacture Factory Price.Wood Fireplace Double Skin Chimney Flue Pipes, 175-184[600d Marine Grade Polyester Canvas Trailerable Waterproof Boat Cover]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap]

Whitaker,J.R.(1963),High Quality 304 Stainless Steel Sheet for Home Decoration Material. Analytical Chemistry35(12), 1950-1953[Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Plastic Plating Makeup Brush SetAuthor for correspondence