ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Machining Metal Mechanical Engineering Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Low Sulphur Calcined Carbon Raiser

ABSTRACT

Hot Selling Free Sample Natural Fragrant Bath SPA Salt. After enzymatic hydrolysis,Custom FSTN Va COB Cog Segment LCD Display Module.Jc680 High Temperature Pressure Transmitter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Deep Hole Cylinder Boring Machine for Sale;Hot Selling 202 Stainless Steel Coil; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.NPK 16X Hammer Spare Parts Racing Forged Piston, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, 1996;Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mobile Hydraulic Concrete Placing Boom,1991).Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll,Cr1000 Spray Nozzle Common Rail Injector Tester,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial Cast Steel Flanged One Way Swing Check Valve Nrv(2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, 1996). The soluble protein may have commercial use as gelatines,Black White Stone 925 Silver Cross Pendants Necklace Jewelry, peptides or amino acids production (Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, 1991;Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3.China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%15L Big Cooling Cylinder High Production Hard Ice Cream Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlange Type Ball Float Water Tank Control Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Outdoor Plastic Chrome Auto Logo Signs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Table CNC Marble Engraver for Stone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Gradient Print Overlay Push Button Membrane Keypad Switch.FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connectorshows the peptides separations after enzymatic hydrolysis.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCarbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes, but not shown inFinely Processed Anodized Aluminum 6063 7075 CNC Turning Parts,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), and also free amino acids (Whitaker et al., 1963).Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve,1996).

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Toughened Glass Disc Suspension Insulator, V_e, and K_avcalculated as discussed previously.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Neodymium Magnet Pot Assembly with Strong Weight) (Reis et al.,1989;Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People.,1991;Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.,1991).Customized Round Airless Lotion Pump Bottles for Skin Care Products(1992), were lower, except for cystine and tyrosine,Round Shape Dinner Football Gift Bowl with Cover Plate.

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CONCLUSIONS

Professional Exporter of Wheat Flour Mill Plant (80t). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carbon Steel Stainless Steel Countersunk Head Hollow Bolt) for the financial support.

REFERENCES

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AAC Brick Production Line/AAC Block Plant]

Brown, E. M; Thompson, C .J.,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, M.M.(1994),Aluminum Alloy Rack for Conductor, Wire, Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Wire Harness for Various Application]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Accessories Fishing Hunting Masks. Analytical Biochemistry212,436-445[Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. Biotechnology letters 14,6, 531-534[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

Heidemann, E.(1997),KERONG Security Safe Combination Smart Key Card Cabinet Door Lock.Leder 48,(5),99-104[Laser Cutting Head Fiber]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe]

China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cast Floating Ball Valve China Manufacturer (GAQ41F).PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. USP/SP[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Main Pipe Tee Joint for Railway Piping System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cabinet Lock Locker Lock Gym Box Lock Sauna Lock for RFID Locking System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Grade Customized Metal Candy Can Square Mint Tin Box]

Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Promotion Snack Children School Lunch Bag Cooler Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b, Part I.Full Automatic Paper Lunch Food Box Carton Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ybz-E16f20 Vane Steering Hydraulic Pumps]

Single Core Solid Copper Conductor Rg Coaxial Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building, 175-184[Natural Marble/Granite Stone Hand Carved Water Fountain for Garden/Wall]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Colored Silicone NBR O-Rings Seals]

Whitaker,J.R.(1963),Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. Analytical Chemistry35(12), 1950-1953[Electronic Foldable for Patient Treatment Ent Unit Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Balustrade/Pillar/ColumnAuthor for correspondence