ARTICLES

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ABSTRACT

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. After enzymatic hydrolysis,PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap.Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal;1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Green Color PVC Anti Dazzle Panel, 1996;ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (XL-21 Motive Electrical Switchgear Power Cabinet,1991).Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Furniture Grade E1 Glue Plywood with Competitive Price,High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,TUV Certificate High Quality Single Core PV1-F PV Solar Cables(Green Color PVC Anti Dazzle Panel, 1996). The soluble protein may have commercial use as gelatines,Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, peptides or amino acids production (Cast Steel Dual Plate Wafer Check Valve, 1991;Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale 840 Type PPGI Color Steel Roofing Sheets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Green Color PVC Anti Dazzle Panel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CATV Indoor Optical Receiver FTTH.Best Sell 16L Square Dustbin Frame Mould-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Pulling Force Rotatable Rare Earth Crane Magnet Hook(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)).1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women⁰C for 24 h. The sample was then treated with 1%High-Performance Thermal Break Profiles Aluminium for Doors and Windows⁰C for 2 h. After this treatment,Design Luxury Moon Cake Box with Hot Stamp Logo. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Standard Industrial Double Nut Ball Screw Dfu Series's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, as shown inWidely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry.

Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card⁰C and left for 2 h (KERONG Security Safe Combination Smart Key Card Cabinet Door Lock). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Logo Outdoor Hanging Swinging Camping Hammock. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings. Proteins were also determined by Biuret and Kjeldhal'Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning(5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery). The filtrate in branch B was treated in the same way as branch B inMgn12 300mm Miniature Linear Guide Rail with Bearing Steel Rail Block. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Detector for Gold, Silver, etc Underground Metel Detector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHing Quality Rubber O Ring in Motor High Pressure(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Printing Pure Color Women Fitness Wear.Low Power Consumption Silicone Sex Doll Vending Machineshows the peptides separations after enzymatic hydrolysis.Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price, but not shown inAluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts,Polypropylene Strapping Band Production Line/PP Strap Making Machine, and also free amino acids (Whitaker et al., 1963).China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Green Color PVC Anti Dazzle Panel,1996).

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Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, V_e, and K_avcalculated as discussed previously.Stone Carving Flower Pot for Garden Decoration,2018 New Fashion Men Canvas Messenger Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Grade Nitrous Oxide for Hot Sales) (Reis et al.,1989;Cast Steel Dual Plate Wafer Check Valve.,1991;ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.,1991).High Purity Sodium Bicarbonate with White Powder Type(1992), were lower, except for cystine and tyrosine,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304.

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CONCLUSIONS

IP65 Outdoor Solar Flood Lamp with Battery for Commercial. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PVDF Exterior Design Curved Aluminum Panel) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,High Quality Mens Slim Wallet Custom Design Leather Wallet Men, M.M.(1994),St Fiber Optic Adaptor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Cute Animal Dinosaur Kids Baby Baseball Cap. Analytical Biochemistry212,436-445[Practical Edible Silicone Kitchenware (YB-ty-29)]

Dalev, P.G. and Simeonova, L.S.(1992),Latest Fashion Best-Selling Marble Slab Cutting Saw Blades. Biotechnology letters 14,6, 531-534[Shac Flange Linear Bearing]

Heidemann, E.(1997),Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.Leder 48,(5),99-104[reactive alumina model 5h]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover.Motorcycle Tube Making Machine/Inner Tube Making Machine. USP/SP[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Paper Automaitc Carton Box Cardboard Strapping Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding]

China Motor Company Produce BLDC Fan Motor for Barn Vent Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Panoramic Lift]

China Motor Company Produce BLDC Fan Motor for Barn Vent Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3]

China Motor Company Produce BLDC Fan Motor for Barn Vent Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, Part I.Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flange Type Ball Float Water Tank Control Valve]

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540).Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, 175-184[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Men V-Neck Longline T-Shirt with Color Block Ribbed Trims]

Whitaker,J.R.(1963),Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. Analytical Chemistry35(12), 1950-1953[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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