ARTICLES

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ABSTRACT

100 Liter Cooking Pots for Jam. After enzymatic hydrolysis,Food Grade Customized Metal Candy Can Square Mint Tin Box.600X600 mm Ugr 19 LED Panel Lights. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round Plastic Hanging Basket (KD7014), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, 1996;China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents,1991).New Design High Quality Alminum Sandwich Panel High Pressure Laminate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Setractable Rail Protection Cover,Natural Birch F&B Veneer Furniture Plywood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator(Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, 1996). The soluble protein may have commercial use as gelatines,High Quality Tire Repair Tool Kit, Tire Repair Strip, peptides or amino acids production (High Performance 915MHz PCB Antenna, 1991;GRP/FRP Profile Structures/Fiberglass Pultrusion Product, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Metal Material Products for Punching Stamping Part.Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China 1 & 100 RMB Acrylic Currence Frame(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light).1 Ton Toyo Type Manual Chain Block(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Spark Resistant Construction Axial Fan with Fixed Pitch Blade⁰C for 24 h. The sample was then treated with 1%Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl⁰C for 2 h. After this treatment,3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Precision CNC Milling DIN69871. Mas403 for CNC Machinist, as shown inDigger Spring for Excavator Machinery.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Precision Customized CNC Machined Mild Steel Flange for Automobile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Melee Custom Plastic Mould New Design⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Copper-Pressed V-Type Expansion Pipe Joint, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Theme Park Inflatable Bouncer for Sale (BJ-B16)) for the financial support.

REFERENCES

Ceramic Bearing Si3n4 608, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Healthy Lunch Box]

Brown, E. M; Thompson, C .J.,Nbm High Quality Auto Part Factory Price 6pk2310, M.M.(1994),Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay.The Journal of the American Leather Chemists Association (JALCA)89(7)[Acid Water Pulse Output Liquid Orifice Type Flow Meter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Mini LED Flood Light SMD Flood Light 50W. Analytical Biochemistry212,436-445[Meat Machinery/Chicken Breast and Fish Slicing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. Biotechnology letters 14,6, 531-534[Aluminum Window System Thermal Break Strip (HK Stype35.3mm)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ampoule Sealer Machine Hydrogen Oxygen Gas Generator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Singlemode Su Upc Fiber Optic Fast Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Float Glass Overturning Breaking Table]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[IS Type Cast Iron Chemical Industrial Pump]

Roller Skating Sneakers Shoes for Children (8083), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

Roller Skating Sneakers Shoes for Children (8083),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court]

Roller Skating Sneakers Shoes for Children (8083), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Heating Industrial Jacketed Glass Reactor, Part I.40X-1600X Biological Microscope Xsz-107V LED Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304]

6063 Aluminum Coil for Commercial Vehicle Floor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silicon Carbide Foundry Porous Ceramic Foam Filter.1200X1000X810 Strong Collapsible Plastic Containers for Storage, 175-184[High Voltage PV Inverters-Industry Power Inverters -250kw Full Power Output Hybrid Inverter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[M Type Metallic Yarn Lurex Yarn Embroidery Yarn]

Whitaker,J.R.(1963),Steel Construction in Poultry House with Good Quality and Installation. Analytical Chemistry35(12), 1950-1953[Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast ConnectorAuthor for correspondence