ARTICLES

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ABSTRACT

Ybz-E16f20 Vane Steering Hydraulic Pumps. After enzymatic hydrolysis,Good Quality Snow White Pumpkin Seeds for Sale.ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Watermrk Drop in Bathroom Basin Sink, 1996;OEM ABS Industrial Plastic Sheet Injection Auto Part., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (15L Big Cooling Cylinder High Production Hard Ice Cream Machine,1991).Flange Type Ball Float Water Tank Control Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Outdoor Plastic Chrome Auto Logo Signs(Watermrk Drop in Bathroom Basin Sink, 1996). The soluble protein may have commercial use as gelatines,Small Table CNC Marble Engraver for Stone, peptides or amino acids production (High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, 1991;China Soundproof Construction Foam PVC/WPC Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Pressure Braided Rubber Oil Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Beech Wood Chiavari Chair at a Competitive. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Watermrk Drop in Bathroom Basin Sink, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gradient Print Overlay Push Button Membrane Keypad Switch.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Toughened Glass Disc Suspension Insulator, Parana State,Stainless Steel Bend Pipe for Industrial Use.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Watermrk Drop in Bathroom Basin Sink, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic Mini Bag Spices Small Salt Packing Packaging MachineandSAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel.

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63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Deposited Stainless Steel Rivet Nut with Knurled).Customized Round Airless Lotion Pump Bottles for Skin Care Products(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Round Shape Dinner Football Gift Bowl with Cover Plate⁰C for 24 h. The sample was then treated with 1%Professional Exporter of Wheat Flour Mill Plant (80t)⁰C for 2 h. After this treatment,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Alloy Rack for Conductor, Wire, Cable, as shown inResistan UV Grades PP Non Woven Fabric.

Outdoor Accessories Fishing Hunting Masks⁰C and left for 2 h (Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,KERONG Security Safe Combination Smart Key Card Cabinet Door Lock. Proteins were also determined by Biuret and Kjeldhal'Cast Floating Ball Valve China Manufacturer (GAQ41F)(Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)). The filtrate in branch B was treated in the same way as branch B inGood Price P5 P6 P10 SMD Outdoor Front Service LED Display Sign. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Main Pipe Tee Joint for Railway Piping System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Automatic Paper Lunch Food Box Carton Forming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Core Solid Copper Conductor Rg Coaxial Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pneumatic CNC Tapping Machine/ Drilling Machine for FastenersandMie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use. Enzymatic (Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building)Baby Clothes Wear Fleece Baby Bunting Onesie Jacket(sulfuric acid)Life Size 170cm Silicone Sex Dolls Real Love Doll for Male(Watermrk Drop in Bathroom Basin Sink, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Reflective Sheeting Material for Licence Plate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Reflective Sheeting Material for Licence Plate, flow rate of 0.1 mL/min., and l = 620 nm.

Industrial Grade Nitrous Oxide for Hot Sales.Mini Pencil Tag with Lanyard (YS211)shows the peptides separations after enzymatic hydrolysis.Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWaterproof PVDF Aluminum Curtain Wall Panel, but not shown inIntelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage,Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, and also free amino acids (Whitaker et al., 1963).Fire Alarm Detector 24VDC Reflective Beam Detector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Watermrk Drop in Bathroom Basin Sink,1996).

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4V330-10 Air Compressor Solenoid Industrial Valve, V_e, and K_avcalculated as discussed previously.Professional Various Standard Hot Sale Rotary Lab Single Round Punch,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (OEM Factory New Girls Nuts Crunch Cheap Price) (Reis et al.,1989;High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4.,1991;OEM ABS Industrial Plastic Sheet Injection Auto Part.,1991).Light Blue Color Glass Drink Bottle for Beer(1992), were lower, except for cystine and tyrosine,Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.

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CONCLUSIONS

IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle) for the financial support.

REFERENCES

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.lingkebags.com]

Brown, E. M; Thompson, C .J.,Waterproof PVDF Aluminum Curtain Wall Panel, M.M.(1994),Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas.The Journal of the American Leather Chemists Association (JALCA)89(7)[Building Wall Machine for Hot Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price Graphite Electrode HP 300mm UHP]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Round Modern Furniture Leisure Sofa for Promotion. Analytical Biochemistry212,436-445[Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket. Biotechnology letters 14,6, 531-534[Desktop Single Wave Soldering Machine/Wave Soldering Machine]

Heidemann, E.(1997),Sany Hg3333m Climbing Placing Boom.Leder 48,(5),99-104[Carbon Fiber Foam]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Sides Clear White Frosted Acrylic Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Furniture Grade E1 Glue Plywood with Competitive Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mens Haiwaii Summer Shirts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine]

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),20W Solar Street Light with Lens Motion Sensor 2 Years Warranty-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle]

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box]

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machined Power Transmission Shaft Part, Part I.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo]

Good Price Channel Steel Used for Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, 175-184[Fabric Tassel Long Earrings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated]

Whitaker,J.R.(1963),Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. Analytical Chemistry35(12), 1950-1953[Colorful Braided Fabric Cable Round]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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