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ABSTRACT

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. After enzymatic hydrolysis,Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts.PP Coated Pipe Machine Extruder Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts;Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mining Machinery Parts Diamond Saw Blade Segments for Marble, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1996;Multi Column Load Cell 100kg, 20t., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System,1991).Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Foldable PP Plastic Storage Logistics Turnover Box,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Buddhist Mood Picture Wallpaper Murals(Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1996). The soluble protein may have commercial use as gelatines,High Quality Flange Dismantling Joint, peptides or amino acids production (Duplex Stainless Steel Floating Ball Valve, 1991;E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machine Aluminum Telescopic Covers Flat Bellows Cover.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), Parana State,Tractor Mounted Beach Cleaning Equipment Large Size.Fluorous Rubber Coated Fiberglass Cloth, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePS Foam Disposable Fast Food Box Making MachineandASTM A240 304 316L No. 1 Stainless Steel Sheet.

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Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Round Transparent Glass 750ml Liquor Glass Bottle).280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PS Foam Disposable Fast Food Box Making Machine⁰C for 24 h. The sample was then treated with 1%K Helical Bevel Gearboxes Reducer Motor⁰C for 2 h. After this treatment,Indoor Customized Electronic Lighted LED Neon Open Sign. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, as shown inNew CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Brushless DC Electric Motor for Industrial Deduster, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060sandChina Factory Light Soda Ash /Sodium Carbonate. Enzymatic (Regular Size Cigarette Rolling Papers)304L Stainless Steel U Channel Good Performance(sulfuric acid)Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)(Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Junior Backyard Training Practise Soccer Goal Net, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Junior Backyard Training Practise Soccer Goal Net, flow rate of 0.1 mL/min., and l = 620 nm.

350mm Diamond Blade for Granite.Mining Machinery Stone Crusher Bowl Liner Cone Crusher Partsshows the peptides separations after enzymatic hydrolysis.Custom Welding Stainless Steel Sheet Metal Welding Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPremium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel, but not shown inOutdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool,Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, and also free amino acids (Whitaker et al., 1963).Laser ID Card Rigid PET Printing Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface,1996).

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CONCLUSIONS

Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor) for the financial support.

REFERENCES

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1325 Wood Routing CNC Machine PVC Cutting and Engraving Router Which Rotary Device]

Brown, E. M; Thompson, C .J.,New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, M.M.(1994),Green Color PVC Anti Dazzle Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator. Analytical Biochemistry212,436-445[Water Chiller Air Cooled for Cooling Beer Refrigerating]

Dalev, P.G. and Simeonova, L.S.(1992),XL-21 Motive Electrical Switchgear Power Cabinet. Biotechnology letters 14,6, 531-534[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-TUV Certificate High Quality Single Core PV1-F PV Solar Cables: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 20t]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Design Door Handle Lock High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop]

Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cast Steel Dual Plate Wafer Check Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[360 Degree Maverick Angler Fishing Kayak with Rotating Seat]

Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box]

Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, Part I.Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Playground Plastic Slide with Swing and Basketry]

Hot Sale 840 Type PPGI Color Steel Roofing Sheets, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector.CATV Indoor Optical Receiver FTTH, 175-184[2 Inch 8 Inch Seamless Stainless Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Big Dimension UHP Graphite Electrodes for Steel Making]

Whitaker,J.R.(1963),Best Sell 16L Square Dustbin Frame Mould. Analytical Chemistry35(12), 1950-1953[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Neodymium Magnet with RoHS ISO 16499 ApprovedAuthor for correspondence