ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,100 Liter Cooking Pots for Jam

ABSTRACT

Food Grade Customized Metal Candy Can Square Mint Tin Box. After enzymatic hydrolysis,600X600 mm Ugr 19 LED Panel Lights.Good Quality Hot Tube8 Japanese Stainless Steel Tube 666. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine;Round Plastic Hanging Basket (KD7014); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, 1996;10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design High Quality Alminum Sandwich Panel High Pressure Laminate,1991).Steel Setractable Rail Protection Cover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Natural Birch F&B Veneer Furniture Plywood,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Tire Repair Tool Kit, Tire Repair Strip(China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, 1996). The soluble protein may have commercial use as gelatines,High Performance 915MHz PCB Antenna, peptides or amino acids production (GRP/FRP Profile Structures/Fiberglass Pultrusion Product, 1991;Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Metal Material Products for Punching Stamping Part. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, Parana State,100 Liter Cooking Pots for Jam.China 1 & 100 RMB Acrylic Currence Frame, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Mens Slim Wallet Custom Design Leather Wallet MenandCommercial Inflatable Dry Slide for Kids.

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1 Ton Toyo Type Manual Chain Block(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Melamine/Plain Chipboard).Spark Resistant Construction Axial Fan with Fixed Pitch Blade(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl⁰C for 24 h. The sample was then treated with 1%3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home⁰C for 2 h. After this treatment,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Precision CNC Milling DIN69871. Mas403 for CNC Machinist's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, as shown inTtp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.

Building Material of Granite Ceramic Wall Tile for Wall Decoration⁰C and left for 2 h (Painted G80 Cargo Lashing Chain with Hooks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. Proteins were also determined by Biuret and Kjeldhal'High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop(GRP/FRP Profile Structures/Fiberglass Pultrusion Product). The filtrate in branch B was treated in the same way as branch B inAcid Chrome Black T Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Precision Customized CNC Machined Mild Steel Flange for Automobile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Container Packaging for Edible Oil Tin Cans Storage Boxes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Copper-Pressed V-Type Expansion Pipe Joint, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming MachineandWhite Desiccant Bag Silica Gel with Indicated Orange Bead. Enzymatic (High Quality Iron Wire Mesh Chain Link Mesh Security Fence)TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump(sulfuric acid)5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide(China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Ceramic Bearing Si3n4 608. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Muliti-Size Light Hot Stamping Foil for PS Frame) for the financial support.

REFERENCES

Nbm High Quality Auto Part Factory Price 6pk2310, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ice cream cart in Dubai]

Brown, E. M; Thompson, C .J.,Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay, M.M.(1994),High Quality Mini LED Flood Light SMD Flood Light 50W.The Journal of the American Leather Chemists Association (JALCA)89(7)[70L Lab Use Refrigerated Circulator Chiller Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Duplex Stainless Steel Floating Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. Analytical Biochemistry212,436-445[Flexible Vj Rigid Shaft Coupling for Pipe Flange]

Dalev, P.G. and Simeonova, L.S.(1992),Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass. Biotechnology letters 14,6, 531-534[10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents]

Heidemann, E.(1997),Professional Manufacture Metal Medal Promotional Gift.Leder 48,(5),99-104[12 Strand Braided Synthetic UHMWPE/Hmpe Rope Towing Rope Manufactory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mashroom Head Nylon Anchor with Pozi Nail]

SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving.Singlemode Su Upc Fiber Optic Fast Connector. USP/SP[Muliti-Size Light Hot Stamping Foil for PS Frame]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Roller Skating Sneakers Shoes for Children (8083): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[K20 Tungsten Carbide Strips]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 6063]

Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Heating Industrial Jacketed Glass Reactor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Baby Clothes Wear Fleece Baby Bunting Onesie Jacket]

Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Rapid Prototype Leather Logo Stamping Mould]

Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:40X-1600X Biological Microscope Xsz-107V LED Light, Part I.6063 Aluminum Coil for Commercial Vehicle Floor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bolts and Nuts Set Brass Fasteners Double Hex Bolt]

Silicon Carbide Foundry Porous Ceramic Foam Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1200X1000X810 Strong Collapsible Plastic Containers for Storage.Steel Construction in Poultry House with Good Quality and Installation, 175-184[Waterproof Motorcycle Duffle Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Kubota Combine Harvester Spare Parts 5h484-2392 Nut]

Whitaker,J.R.(1963),Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White. Analytical Chemistry35(12), 1950-1953[Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pet Short Fiber Non Woven Geotextile for Landfill FiltrationAuthor for correspondence