ARTICLES

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ABSTRACT

Portable Powerful Charger with Dual Pd Charger and Type-C Charger. After enzymatic hydrolysis,Flexible Vj Rigid Shaft Coupling for Pipe Flange.Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price, 1996;15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Speed Steel Mill Roll with High Quality,1991).3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2017 China Hot Sale Water Screen Movie Fountain,Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor(100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price, 1996). The soluble protein may have commercial use as gelatines,LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, peptides or amino acids production (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , 1991;Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Latest New Hho Engine Oil Carbon Remover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wood Chips Rotary Dryer Sawdust Pellet Drying Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Delicate Handmade Round Natural Stone Chain Necklace. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price.Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Embossed Aluminium/ Aluminum Household Foil 8011-O(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Full Color Indoor Outdoor LED Curtain TV Display, Parana State,Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts.High Quality Plastic Injection Mold Parts Square Interlocks Maker, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashionable Natural Bamboo Wood Sunglasses for Menand8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial.

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LED Outdoor Solar Lawn Garden Lighting for Wall(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)).3-Pin Australia Power Cord Plug with SAA(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sludge Oil Separation Centrifuge⁰C for 24 h. The sample was then treated with 1%8011 Aluminum Foil for Cap Used⁰C for 2 h. After this treatment,Pepper Peanut Sauce Colliod Miller Grinding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gasoline Rototiller, Cultivator, Tiller Model Gtm-6's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, as shown inMultifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.

Electronic Foldable for Patient Treatment Ent Unit Chair⁰C and left for 2 h (Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Pushbutton Ultrasonic Spin Welding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles. Proteins were also determined by Biuret and Kjeldhal'Double Shaft Paper Cardboard Waste Plastic Shredder Machine(Stainless Steel DIN7981 Pan Head Self Tapping Screw). The filtrate in branch B was treated in the same way as branch B inElectrostatic Powder Coating Spraying Equipment (Colo-800D). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Desktop Single Wave Soldering Machine/Wave Soldering Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacturers White Dinnerware Set Fast Food Tray Bento Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wld8200 Auto Spray Paint Booth, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Outdoor Playground Plastic Slide with Swing and Basketry. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cross Hex Flange Screw) for the financial support.

REFERENCES

Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Closed Box-Type Mobile File-Intensive Racking System]

Brown, E. M; Thompson, C .J.,Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, M.M.(1994),Belt Conveyor Systmem for Fertilizer Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Quality Heavy Duty Globe Control Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price. Analytical Biochemistry212,436-445[Q3210 Vibratory Polishing Shot Blasting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Digger Spring for Excavator Machinery. Biotechnology letters 14,6, 531-534[Building Decoration Material Acrylic Dispersant for Coating]

Heidemann, E.(1997),1.8ml Clear Sample Vials with Label and PP Caps.Leder 48,(5),99-104[1.8mm One Way Vision Mesh Dva Mesh]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Line Scan Uvss System Portable Under Vehicle Surveillance]

Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Small Coil Compression Spring in High Quality.Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates. USP/SP[Customized Logo Outdoor Hanging Swinging Camping Hammock]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Supply Straight Ball Joint Bearing 02b04]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.hengyaomachinery.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

Plastic Material Handling Dolly Platform Hand Trolley Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High-Tower Compound NPK Fertilizer 15-15-15]

Plastic Material Handling Dolly Platform Hand Trolley Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4.0MP Ahd Tvi CVBS HD CCTV Camera Module]

Plastic Material Handling Dolly Platform Hand Trolley Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes, Part I.99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Precision Ball Screw with Two Nut]

ATV Spiked Chain Harrow for Farming, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60.Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody, 175-184[Amazon Professional Electric Trimmer Shell Retro Push-Shear Shell]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wood Fireplace Double Skin Chimney Flue Pipes]

Whitaker,J.R.(1963),Fashionable Natural Bamboo Wood Sunglasses for Men. Analytical Chemistry35(12), 1950-1953[12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Screw MachineAuthor for correspondence