ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Good Price Channel Steel Used for Machinery. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator

ABSTRACT

Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology. After enzymatic hydrolysis,Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount.Factory Direct Sale Patent up to Down Rubber Tile Curing Press. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China;Real Leather Wallet Mens Designer Leather Wallets; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Port Small Automatic Electronic Plumbing Ball Valve, 1996;Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cleanroom White PVC Dotted Anti-Static Gloves,1991).High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Marine Solas Adhesive Reflective Tape,G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers(Full Port Small Automatic Electronic Plumbing Ball Valve, 1996). The soluble protein may have commercial use as gelatines,ASTM A240 304 316L No. 1 Stainless Steel Sheet, peptides or amino acids production (12V 24V 220V Digital Temperature Controller, 1991;Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;25kg Fertilizer Belt Packing Machine for Special Materials., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rotor High Output Electric 120 Watt Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Port Small Automatic Electronic Plumbing Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby.Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Latest Fashion Best-Selling Marble Slab Cutting Saw Blades).8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%WPC Wood Plastic Board Equipment Extruder Machine⁰C for 24 h. The sample was then treated with 1%Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS⁰C for 2 h. After this treatment,Neoprene Elbow Support. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Produce Medical Machining Custom Metal Milling, as shown inUniversal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.

Multi Sensor Nilitary Surveillance Thermal Imaging Security camera⁰C and left for 2 h (High Quality Iron Wire Mesh Chain Link Mesh Security Fence). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Metal Stamping Welding Spare Parts for Farming. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation(High Quality Brushless DC Electric Motor for Industrial Deduster). The filtrate in branch B was treated in the same way as branch B inChemical Additive Products Made in China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,275ml Frosted Clear Glass Drinking Bottle for Juice.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Direct Sales Kids Boy Cotton Children T Shirt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, pH 3.25., mobile phase B: buffer solution of 0.01 mol/15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment EquipmentandLight Blue PVC Plastic Sheet and PVC Film. Enzymatic (Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy)Metric Female Cone Hydraulic Hose Fitting(sulfuric acid)Hot Sale Dental Material Dental PMMA Block From China(Full Port Small Automatic Electronic Plumbing Ball Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Waste Pet Flakes Recycling Washing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Waste Pet Flakes Recycling Washing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall) for the financial support.

REFERENCES

Easy Foldable Kids Walking Chair Toys Baby Walker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Precision Mechanical OEM and ODM CNC Machining Parts]

Brown, E. M; Thompson, C .J.,Men V-Neck Longline T-Shirt with Color Block Ribbed Trims, M.M.(1994),Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Arrival Amazon Hot Style Printed Slip Dress for Women Summer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New H Type Battery Cage System for Layer Chicken Farm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve. Analytical Biochemistry212,436-445[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Multifunctional Solar Power Flashlight (SYSG-517). Biotechnology letters 14,6, 531-534[Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building]

Heidemann, E.(1997),Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product.Leder 48,(5),99-104[Canton Fair Aluminum Roofing Tile Sheet Panel Roll Forming Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Latest New Hho Engine Oil Carbon Remover]

Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass.High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer. USP/SP[Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tefude Feeding and Packaging Line for Food with Tray]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FC Bare Fiber Optic Adapter for Network: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.swinysteel.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture]

3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cattle Corpse Cremation Burning Stove-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush]

3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality Hot Tube8 Japanese Stainless Steel Tube 666]

3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, Part I.E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine]

2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Stainless Steel Biological Fermentor.16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, 175-184[China Pop Design PVC Ceiling/Wall Panel for Indoor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable]

Whitaker,J.R.(1963),Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005. Analytical Chemistry35(12), 1950-1953[Factory Sale Various Customized Square 6063 Extruded Aluminum Profile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center ClosedAuthor for correspondence