ARTICLES

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ABSTRACT

Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. After enzymatic hydrolysis,Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal.Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quaity China Supplier Truck Trailer Auto Car Accessories, 1996;Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light,1991).Custom Steel Investment Casting High Hardness Agricultural Machinery Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Minimalist Slim Real Leather Front Pocket Card Wallet,Natural Cleaning Konjak Sponge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indoor Load Break Switch Isolating Switch(High Quaity China Supplier Truck Trailer Auto Car Accessories, 1996). The soluble protein may have commercial use as gelatines,Biodegradable Disposible Straw Pulp Food Box Ppp-1007, peptides or amino acids production (T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, 1991;Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;NBR Latex Adhesive for Glove., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Wooden House Zinc Plated Angle Bracket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quaity China Supplier Truck Trailer Auto Car Accessories, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box.Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage).Longyi Crimping Types Cable Terminal Lugs(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rigid PVC Foam Board for FRP Marine⁰C for 24 h. The sample was then treated with 1%Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display⁰C for 2 h. After this treatment,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Furniture Gold PVD Plasma Coating Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, as shown inCustomized Double Acting Hydraulic Cylinder for Excavator.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Solid Tungsten Carbide Rods, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Duplex Stainless Steel Floating Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12 Inch King Mattress Latex Foam Memory Foam Mattress) for the financial support.

REFERENCES

E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bugle Snacks Food Extruder]

Brown, E. M; Thompson, C .J.,Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, M.M.(1994),Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.The Journal of the American Leather Chemists Association (JALCA)89(7)[Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt. Analytical Biochemistry212,436-445[Good Quality Snow White Pumpkin Seeds for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. Biotechnology letters 14,6, 531-534[8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight]

Heidemann, E.(1997),7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input.Leder 48,(5),99-104[Retarded-Releasing Concrete Water Reducing Agent--PCE for Concrete Admixtures]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cold Drawn D2 H13 Alloy Steel Turning Roll]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chrysoidine Crystals Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Metal Polishing Disc]

HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steel Floor Deck Post Cutting Cold Roll Forming Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Container House for Clinic or Hospital]

HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20W Solar Street Light with Lens Motion Sensor 2 Years Warranty]

HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), Part I.Fluorous Rubber Coated Fiberglass Cloth. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern).PS Foam Disposable Fast Food Box Making Machine, 175-184[Corrosion Resistant Vertical Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

Whitaker,J.R.(1963),K Helical Bevel Gearboxes Reducer Motor. Analytical Chemistry35(12), 1950-1953[Competitive Price Sodium Gluconate as Concrete Admixture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cr1000 Spray Nozzle Common Rail Injector TesterAuthor for correspondence