ARTICLES

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ABSTRACT

Lifting Thread with Needle for Plastic Surgery. After enzymatic hydrolysis,Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls.LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fudan Compatible Card 1k RFID Card 13.56MHz;Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Biodegradable Food Packaging Take out Food Boxes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, 1996;Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring,1991).20 Ton Small Vertical Plastic Injection Molding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White Hand Carved Angel Sculpture, Marble Child Statue for Garden,Foska Mini Size High Quality Heavy Duty Stapler,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5.5X19mm Hex Head Self Tapping Screws with Washer(Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, 1996). The soluble protein may have commercial use as gelatines,Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose, peptides or amino acids production (Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding, 1991;24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Plastic Plating Makeup Brush Set., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Road Construction Non Woven Needle Punched PP Non Woven Geotextile.Wholesale Scented Flavor Flower Herbal Tea-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Organic Fertilizer Compost Turning Machine, Compost Turner Machine, Parana State,Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable.Insulation Top Hung Aluminum Awning Window with Mosquito Net, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSimilar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)andCNC Machining Metal Mechanical Engineering Parts.

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4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sell Data USB Connector Type C 12 Pin Terminal Block).Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sell Data USB Connector Type C 12 Pin Terminal Block⁰C for 24 h. The sample was then treated with 1%Unique Handle 24mm Water Bottle Caps⁰C for 2 h. After this treatment,Stainless Steel Band Cable Ties Wire Strap Steel Banding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Acid Water Pulse Output Liquid Orifice Type Flow Meter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware, as shown in50g Body Luxury King Crown Cosmetic Containers.

Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible⁰C and left for 2 h (2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment. Proteins were also determined by Biuret and Kjeldhal'High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production(20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21). The filtrate in branch B was treated in the same way as branch B inExtruder Barrel Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Door Heated Holding Carts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 New Water Quality TDS Test, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Furniture Hotel Dining Furniture Metal Hotel Table Chair, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Precision Mold Manufacturer Plastic Injection MouldandContainer House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital. Enzymatic (Machinery Parts Aeroplane Engine CNC Milling)Aws E7018 Low Carbon Steel Welding Electrode(sulfuric acid)Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting(Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Electrical Bike Waterproof Power Connector, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Electrical Bike Waterproof Power Connector, flow rate of 0.1 mL/min., and l = 620 nm.

Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass.WPC Wood Plastic Board Equipment Extruder Machineshows the peptides separations after enzymatic hydrolysis.Painted G80 Cargo Lashing Chain with Hooks(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in40X-1600X Biological Microscope Xsz-107V LED Light, but not shown in19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS,Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall, and also free amino acids (Whitaker et al., 1963).Automatic Feeding Customized Multi Layers Shirts Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer,1996).

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Tire Repair Stripand400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Makingshow the gel chromatographic separation of soluble protein with Sephadex G-Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts.Portable Powerful Charger with Dual Pd Charger and Type-C Charger, and another peak at 45 mL not shown inAcid Water Pulse Output Liquid Orifice Type Flow Meter, for peptides lower than 3000 Da.Flexible Vj Rigid Shaft Coupling for Pipe Flange(Condell et al.,1993) .

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CONCLUSIONS

15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4.0MP Ahd Tvi CVBS HD CCTV Camera Module) for the financial support.

REFERENCES

High Speed Steel Mill Roll with High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Oxygen Plant for Cutting Pressure Swing Adsorption Plant Oxygen Machine]

Brown, E. M; Thompson, C .J.,3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, M.M.(1994),2017 China Hot Sale Water Screen Movie Fountain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Die Casting Zinc Alloy Badge Use for Car]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ampoule Sealer Machine Hydrogen Oxygen Gas Generator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes. Analytical Biochemistry212,436-445[55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk]

Dalev, P.G. and Simeonova, L.S.(1992),Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. Biotechnology letters 14,6, 531-534[IS Type Cast Iron Chemical Industrial Pump]

Heidemann, E.(1997),LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip.Leder 48,(5),99-104[Low Cost Thin Blade Slitter Scorer Machine for Carton Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Latest New Hho Engine Oil Carbon Remover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[M Type Metallic Yarn Lurex Yarn Embroidery Yarn]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stud with Reflector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve]

Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Delicate Handmade Round Natural Stone Chain Necklace-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

Wood Chips Rotary Dryer Sawdust Pellet Drying Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, Part I.Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

Embossed Aluminium/ Aluminum Household Foil 8011-O, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021).Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, 175-184[CE Approved 65*15mm Disposable Plastic Culture Petri Dish]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Eye Anti-Aging Serum]

Whitaker,J.R.(1963),Cross Hex Flange Screw. Analytical Chemistry35(12), 1950-1953[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display ScreenAuthor for correspondence