ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Speed Steel Mill Roll with High Quality. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer

ABSTRACT

2017 China Hot Sale Water Screen Movie Fountain. After enzymatic hydrolysis,Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Latest New Hho Engine Oil Carbon Remover, 1996;Wood Chips Rotary Dryer Sawdust Pellet Drying Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Delicate Handmade Round Natural Stone Chain Necklace,1991).Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light,Embossed Aluminium/ Aluminum Household Foil 8011-O,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)(Latest New Hho Engine Oil Carbon Remover, 1996). The soluble protein may have commercial use as gelatines,Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, peptides or amino acids production (Cross Hex Flange Screw, 1991;Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Color Indoor Outdoor LED Curtain TV Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Plastic Injection Mold Parts Square Interlocks Maker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Outdoor Solar Lawn Garden Lighting for Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Latest New Hho Engine Oil Carbon Remover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3-Pin Australia Power Cord Plug with SAA.Sludge Oil Separation Centrifuge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8011 Aluminum Foil for Cap Used(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Laser Cut Sheet Metal Fiber Laser Cutting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Sexy Crystal Self Adhesive Resuable Nipple Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, pH 3.25., mobile phase B: buffer solution of 0.01 mol/130kw 7 Degrees Scroll Type Industrial Water Chiller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Plastic Material Handling Dolly Platform Hand Trolley Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer) for the financial support.

REFERENCES

Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Douling Brand Zsy Series Bevel and Cylindrical Gear Box]

Brown, E. M; Thompson, C .J.,Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes, M.M.(1994),99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5.The Journal of the American Leather Chemists Association (JALCA)89(7)[NPK 16X Hammer Spare Parts Racing Forged Piston]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ATV Spiked Chain Harrow for Farming. Analytical Biochemistry212,436-445[4V330-10 Air Compressor Solenoid Industrial Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60. Biotechnology letters 14,6, 531-534[1 Ton Toyo Type Manual Chain Block]

Heidemann, E.(1997),Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody.Leder 48,(5),99-104[Long Weld Neck Flange]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3bbl Brite Servicing Tank Beer Brewery Equipment Unitank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[130kw 7 Degrees Scroll Type Industrial Water Chiller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Flooring For Wet Areas Supplier & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat]

9~12 Inch Mechanical Backing Plate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Thermal Break Aluminum Profile for Aluminum Windows-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Methyl Cyclohexane Methylcyclohexane]

9~12 Inch Mechanical Backing Plate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sinotruk-HOWO 6X4 Tractor Truck]

9~12 Inch Mechanical Backing Plate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Window System Thermal Break Strip (HK Stype35.3mm), Part I.LED Bulb Light UFO Light Recruitment Distributor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine]

Ozone Generator Plant for Water Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator.Free Assembly, Walking Machine, Home Fitness, Treadmill, 175-184[m.baiyipackage.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light]

Whitaker,J.R.(1963),CMC Transparent Grade for Coating Production. Analytical Chemistry35(12), 1950-1953[Customized Stainless Steel Laser Cutting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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