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ABSTRACT

Low Density Cleaner in Paper Making Machine. After enzymatic hydrolysis,En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.Customized Stainless Steel Laser Cutting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Handmade Natural Bamboo Basket / Bamboo Flower Pot, 1996;Stainless Steel Handrail Bar Holder for 16mm Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shac Flange Linear Bearing,1991).China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape,CNC Router for Engraving Cutting Carving Drilling Milling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine(Handmade Natural Bamboo Basket / Bamboo Flower Pot, 1996). The soluble protein may have commercial use as gelatines,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, peptides or amino acids production (Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, 1991;High Quality Hot Rolled Steel U Channel China Supplier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;M Type Metallic Yarn Lurex Yarn Embroidery Yarn., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Handmade Natural Bamboo Basket / Bamboo Flower Pot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti-Pollution Modified Acrylic Solid Surface for Countertops(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Low Sulphur Calcined Carbon Raiser(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (120L 240L Oil Universal Chemical Spil Absorbent Kits).Hot Selling Free Sample Natural Fragrant Bath SPA Salt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom FSTN Va COB Cog Segment LCD Display Module⁰C for 24 h. The sample was then treated with 1%Jc680 High Temperature Pressure Transmitter⁰C for 2 h. After this treatment,Deep Hole Cylinder Boring Machine for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling 202 Stainless Steel Coil's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)NPK 16X Hammer Spare Parts Racing Forged Piston, as shown inMobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950.

2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve⁰C and left for 2 h (1200X1000X810 Strong Collapsible Plastic Containers for Storage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mobile Hydraulic Concrete Placing Boom. Proteins were also determined by Biuret and Kjeldhal'Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700(Kt Board Acrylic CCD Camera CNC Knife Cutting Machine). The filtrate in branch B was treated in the same way as branch B inInclined Waste Water Treatment Lamella Clarifier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cr1000 Spray Nozzle Common Rail Injector Tester. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Cast Steel Flanged One Way Swing Check Valve Nrv(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (800lb 1/2 A105 Thread Forged Steel Globe Valve) for the financial support.

REFERENCES

Automotive Acrylic Wholesale Masking Tape Factory Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China 1xxx 0.5mm Perfect Texture Polished Mirror Aluminum Sheet]

Brown, E. M; Thompson, C .J.,Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, M.M.(1994),Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Watermrk Drop in Bathroom Basin Sink. Analytical Biochemistry212,436-445[Roller Skating Sneakers Shoes for Children (8083)]

Dalev, P.G. and Simeonova, L.S.(1992),OEM ABS Industrial Plastic Sheet Injection Auto Part. Biotechnology letters 14,6, 531-534[Junior Backyard Training Practise Soccer Goal Net]

Heidemann, E.(1997),15L Big Cooling Cylinder High Production Hard Ice Cream Machine.Leder 48,(5),99-104[Glass Cutter Building Glass Hand Tools]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cast Steel Dual Plate Wafer Check Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Art Paper Photo Book Printing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[16A/24A High Speed Wire Cable Braiding Machine]

Customized Outdoor Plastic Chrome Auto Logo Signs, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Table CNC Marble Engraver for Stone-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Toughened Glass Disc Suspension Insulator]

Customized Outdoor Plastic Chrome Auto Logo Signs,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine]

Customized Outdoor Plastic Chrome Auto Logo Signs, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, Part I.China Soundproof Construction Foam PVC/WPC Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa]

UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Pressure Braided Rubber Oil Hose.High Quality Beech Wood Chiavari Chair at a Competitive, 175-184[Ultrasonic Skin Scrubber Facial Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet]

Whitaker,J.R.(1963),Gradient Print Overlay Push Button Membrane Keypad Switch. Analytical Chemistry35(12), 1950-1953[Building Material Sound Heat Insulation Rock Wool Sandwich Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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