ARTICLES

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ABSTRACT

High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. After enzymatic hydrolysis,100 Liter Cooking Pots for Jam.Food Grade Customized Metal Candy Can Square Mint Tin Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Round Plastic Hanging Basket (KD7014), 1996;Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle,1991).10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design High Quality Alminum Sandwich Panel High Pressure Laminate,Steel Setractable Rail Protection Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Natural Birch F&B Veneer Furniture Plywood(Round Plastic Hanging Basket (KD7014), 1996). The soluble protein may have commercial use as gelatines,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, peptides or amino acids production (High Quality Tire Repair Tool Kit, Tire Repair Strip, 1991;High Performance 915MHz PCB Antenna, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;GRP/FRP Profile Structures/Fiberglass Pultrusion Product., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Round Plastic Hanging Basket (KD7014), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light.Customized Metal Material Products for Punching Stamping Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Muliti-Size Light Hot Stamping Foil for PS Frame, Parana State,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel.Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Round Plastic Hanging Basket (KD7014), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber CuttingandNew Arrival Amazon Hot Style Printed Slip Dress for Women Summer.

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50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple).China 1 & 100 RMB Acrylic Currence Frame(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1 Ton Toyo Type Manual Chain Block⁰C for 24 h. The sample was then treated with 1%Spark Resistant Construction Axial Fan with Fixed Pitch Blade⁰C for 2 h. After this treatment,Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), as shown inAustralia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.

Precision CNC Milling DIN69871. Mas403 for CNC Machinist⁰C and left for 2 h (Latest New Hho Engine Oil Carbon Remover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Building Material of Granite Ceramic Wall Tile for Wall Decoration. Proteins were also determined by Biuret and Kjeldhal'3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine(Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil). The filtrate in branch B was treated in the same way as branch B inTouch Screen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrecision Customized CNC Machined Mild Steel Flange for Automobile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Container Packaging for Edible Oil Tin Cans Storage Boxes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Melee Custom Plastic Mould New Design, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Tefude Feeding and Packaging Line for Food with TrayandCopper-Pressed V-Type Expansion Pipe Joint. Enzymatic (Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber)White Desiccant Bag Silica Gel with Indicated Orange Bead(sulfuric acid)High Quality Iron Wire Mesh Chain Link Mesh Security Fence(Round Plastic Hanging Basket (KD7014), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, flow rate of 0.1 mL/min., and l = 620 nm.

5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide.24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serumshows the peptides separations after enzymatic hydrolysis.500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAmazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, but not shown in2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush,Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, and also free amino acids (Whitaker et al., 1963).360 Degree Maverick Angler Fishing Kayak with Rotating Seat. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Round Plastic Hanging Basket (KD7014),1996).

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CONCLUSIONS

High Quality Crusher for Used Plastic Recycling Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place) for the financial support.

REFERENCES

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[304L 316L Stainless Steel Sheets From Ticso]

Brown, E. M; Thompson, C .J.,Ceramic Bearing Si3n4 608, M.M.(1994),Nbm High Quality Auto Part Factory Price 6pk2310.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. Analytical Biochemistry212,436-445[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Mini LED Flood Light SMD Flood Light 50W. Biotechnology letters 14,6, 531-534[Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot]

Heidemann, E.(1997),Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead.Leder 48,(5),99-104[Mini Water Dispenser Pump]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass.Professional Manufacture Metal Medal Promotional Gift. USP/SP[EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Water Cream Bulk Storage Tank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lady Leather Bags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mexico Single Expansion Anchor with YZP]

Singlemode Su Upc Fiber Optic Fast Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Roller Skating Sneakers Shoes for Children (8083)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet]

Singlemode Su Upc Fiber Optic Fast Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Welded Stud Anchor Chain for Ship]

Singlemode Su Upc Fiber Optic Fast Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, Part I.Electric Heating Industrial Jacketed Glass Reactor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stone Carving Flower Pot for Garden Decoration]

40X-1600X Biological Microscope Xsz-107V LED Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6063 Aluminum Coil for Commercial Vehicle Floor.Silicon Carbide Foundry Porous Ceramic Foam Filter, 175-184[Bluetooth Headset]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Animal Carcass Incinerator Hospital Garbage Incinerator]

Whitaker,J.R.(1963),1200X1000X810 Strong Collapsible Plastic Containers for Storage. Analytical Chemistry35(12), 1950-1953[Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Individually Paper Wrapped Biodegradable Paper Drinking Straws WholesaleAuthor for correspondence