ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Selling Solidwood Viola Wholesale Price Viola. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Building Material Sound Heat Insulation Rock Wool Sandwich Panel

ABSTRACT

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. After enzymatic hydrolysis,Ltmg Classic Product 6 Ton Wheel Front Loader for Sale.Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Household Family Medical Emergency Electronic Digital Thermometer, 1996;Die Cast Iron Worm Gear Speed Reduce Gearbox., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting,1991).High Quality Couple Watches Steel Material Gift Watch OEM in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glass Tea Sugar Coffee Storage Jar with Screw Cap,Circular DC Electro Lifting Magnet for Lifting Scraps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts(Household Family Medical Emergency Electronic Digital Thermometer, 1996). The soluble protein may have commercial use as gelatines,Particle Board Manufacturer, Melamine/Plain Chipboard, peptides or amino acids production (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, 1991;Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-120ml Glass Perfume Bottles for Designer Perfume. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Household Family Medical Emergency Electronic Digital Thermometer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Agriculture Hay Silage Wrapping Film.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromium Oxide Green 99% for Construction and Plastics⁰C and left for 2 h (White Desiccant Bag Silica Gel with Indicated Orange Bead). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zs Round Vibrating Sieve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Tactile Paving Blind Road Stud. Proteins were also determined by Biuret and Kjeldhal'70L Lab Use Refrigerated Circulator Chiller Factory Price(Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper). The filtrate in branch B was treated in the same way as branch B in1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Roller Hearth Heating Furnace for Gas Cylindrer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avcustom design MIFARE DESFire EV2 2K plastic RFID loyalty card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hotel Card Door Lock Access Control Safe Door Lock⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel DIN7981 Pan Head Self Tapping Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (HOWO Tipper Truck Price with Manufacture Factory Price) for the financial support.

REFERENCES

PP/Pet Woven Geotextile for Road Construction, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Transparent PVC Plastic Film Roll for Vacuum Forming]

Brown, E. M; Thompson, C .J.,Lamella Clarifier in Industrial Wastewater Treatment, M.M.(1994),FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering.The Journal of the American Leather Chemists Association (JALCA)89(7)[5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. Analytical Biochemistry212,436-445[Low Power Consumption Silicone Sex Doll Vending Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights. Biotechnology letters 14,6, 531-534[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

Heidemann, E.(1997),Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick.Leder 48,(5),99-104[Saddle PU/PVC Leather]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts]

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts.IS Type Cast Iron Chemical Industrial Pump. USP/SP[CMC Transparent Grade for Coating Production]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Tactile Paving Blind Road Stud]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mango Pulp Juice Processing Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sexy Breathable Heartshaped Running Shorts Yoga Bottom Pants Gym Wear]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Custom Grooved Quartz Plate Deep Processing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale CNC Router Machine for Wood Cutting (1325)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Home Fitness]

Custom Grooved Quartz Plate Deep Processing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industrial Grade Nitrous Oxide for Hot Sales]

Custom Grooved Quartz Plate Deep Processing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, Part I.50g Body Luxury King Crown Cosmetic Containers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot.Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, 175-184[Socket Flange]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tractor Mounted Beach Cleaning Equipment Large Size]

Whitaker,J.R.(1963),4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. Analytical Chemistry35(12), 1950-1953[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500WAuthor for correspondence