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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Wholesale Oxide Aluminium Black Sand Blast Media. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Welded Stud Anchor Chain for Ship

ABSTRACT

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. After enzymatic hydrolysis,Line Scan Uvss System Portable Under Vehicle Surveillance.Folding Steel Manual Wheelchair with Chrome Frame. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel;Fashion Style Lady Alloy Quartz Watch with Simple Dial; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 1996;Building Decoration Material Acrylic Dispersant for Coating., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine,1991).Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supply Straight Ball Joint Bearing 02b04,Wrinkle Effect Crease UV Drying Machine UV Curing System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack(Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 1996). The soluble protein may have commercial use as gelatines,Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, peptides or amino acids production (20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, 1991;Tefude Feeding and Packaging Line for Food with Tray, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CMP No Nc Metal Switch Black 25mm Illuminated Push Button. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Felt Disc Polishing Sanding Disc with Wool Felt Diam(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, Parana State,Welded Stud Anchor Chain for Ship.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theThrough Bolt Assy for Montabert Brh-501 Hydraulic Breaker HammersandDesktop Single Wave Soldering Machine/Wave Soldering Machine.

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Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Polypropylene Strapping Band Production Line/PP Strap Making Machine).Colorful Braided Fabric Cable Round(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Universal Wholesale Hot Sale Custom Blank Keys⁰C for 24 h. The sample was then treated with 1%Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge⁰C for 2 h. After this treatment,Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waw 8 Ton Logistics Light Truck for Transportation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Forging Lathe Spindle Forged Spindle, as shown inDouble Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.

Hot Sale Chic Straw Braid Floppy Hat with Print Tape⁰C and left for 2 h (Furniture Grade E1 Glue Plywood with Competitive Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lamella Clarifier in Sewage Tretament Plant for Water Purification. Proteins were also determined by Biuret and Kjeldhal'BMC (DMC) / SMC Compression Moulding(High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine). The filtrate in branch B was treated in the same way as branch B inHPMC for Cement-Based Tile Adhesives. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ring Caps &Plastic Tube Pop Novelties.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Precision Ball Screw with Two Nut⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Low Density Cleaner in Paper Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Handmade Natural Bamboo Basket / Bamboo Flower Pot.Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Boxshows the peptides separations after enzymatic hydrolysis.Stainless Steel Handrail Bar Holder for 16mm Pipe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, but not shown inChemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated,Shac Flange Linear Bearing, and also free amino acids (Whitaker et al., 1963).China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box,1996).

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T8 20W LED Grow Tube Supplementary Lighting Lamps for PlantandRotor High Output Electric 120 Watt Motorshow the gel chromatographic separation of soluble protein with Sephadex G-Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape.CNC Router for Engraving Cutting Carving Drilling Milling, and another peak at 45 mL not shown in5 Star European Antique Hotel Furniture, for peptides lower than 3000 Da.Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine(Condell et al.,1993) .

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CONCLUSIONS

Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth) for the financial support.

REFERENCES

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Injection Mould for Juice Extractor/ Juicer/ Juicing Machine]

Brown, E. M; Thompson, C .J.,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, M.M.(1994),Anti-Pollution Modified Acrylic Solid Surface for Countertops.The Journal of the American Leather Chemists Association (JALCA)89(7)[2017 China Hot Sale Water Screen Movie Fountain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Expanded Metal Infill Panel Metal Mesh Custom Made]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Analytical Biochemistry212,436-445[Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Corrugated Carton Box Fully Automatic Folding Gluing Machine. Biotechnology letters 14,6, 531-534[ Polished Quartz Disc]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Machining Metal Mechanical Engineering Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Steel Pipe Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Permanent Suspended Magnetic Separator for Belt Conveyor]

Low Sulphur Calcined Carbon Raiser, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Free Sample Natural Fragrant Bath SPA Salt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Carbon Steel Stainless Steel Countersunk Head Hollow Bolt]

Low Sulphur Calcined Carbon Raiser,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap]

Low Sulphur Calcined Carbon Raiser, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom FSTN Va COB Cog Segment LCD Display Module, Part I.Jc680 High Temperature Pressure Transmitter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)]

Deep Hole Cylinder Boring Machine for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Selling 202 Stainless Steel Coil.NPK 16X Hammer Spare Parts Racing Forged Piston, 175-184[Self Adhesive Sealing Strip]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single and Multi Rope Wire Mine Hoist From China]

Whitaker,J.R.(1963),2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. Analytical Chemistry35(12), 1950-1953[1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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