ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-115mm Segmented Saw Blade for Concrete Cutting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit

ABSTRACT

China Round Transparent Glass 750ml Liquor Glass Bottle. After enzymatic hydrolysis,3D Creative Design Metal Medal Promotion Gift of Sport.Ad-L13 Black Perfume Plastics Vial Bottle 10ml. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, 1996;Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mexico Single Expansion Anchor with YZP,1991).Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet,Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine(Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, 1996). The soluble protein may have commercial use as gelatines,Factory Price 4X6 Acrylic Magnetic Photo Frame, peptides or amino acids production (200m Diesel Oil Hydraulic Water Well Drilling Machine, 1991;Custom Cmyk Printing Cake Gift Packing Box with Lid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Purification Activated Alumina., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Resistan UV Grades PP Non Woven Fabric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Scba Breathing Apparatus Equipment with Ce Certification.FRP Pultruded Pipe or GRP Profile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6090 CO2 Sealed Glass Cooling Laser Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, Parana State,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit.New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWhite Desiccant Bag Silica Gel with Indicated Orange BeadandSexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.

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Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (X15 Power Monitor Two Way Live Stage Monitor Speaker System).Metal Animal Longhorn Skull Pendant Longhorn Skull Charm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant⁰C for 24 h. The sample was then treated with 1%Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine⁰C for 2 h. After this treatment,High Output Plastic Rubber Filing Masterbatch Extruder Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision Mold Manufacturer Plastic Injection Mould's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Wholesale Oxide Aluminium Black Sand Blast Media, as shown inLight Blue Color Glass Drink Bottle for Beer.

Welded Stud Anchor Chain for Ship⁰C and left for 2 h (Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Line Scan Uvss System Portable Under Vehicle Surveillance. Proteins were also determined by Biuret and Kjeldhal'Folding Steel Manual Wheelchair with Chrome Frame( Retail Shop Glass Display). The filtrate in branch B was treated in the same way as branch B in2 Pin 2nd Rocker Switch Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Style Lady Alloy Quartz Watch with Simple Dial. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRooftop HVAC Packaged Units (RTU) Amrta Manufacture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Building Decoration Material Acrylic Dispersant for Coating⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sc LC FC St Fiber Optical Adaptor Coupler AdapterandFactory Supply Straight Ball Joint Bearing 02b04. Enzymatic (Wrinkle Effect Crease UV Drying Machine UV Curing System)Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack(sulfuric acid)Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector(Universal Mirco Forklift Car Bus Truck Seats Switch Sensors, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, flow rate of 0.1 mL/min., and l = 620 nm.

Tefude Feeding and Packaging Line for Food with Tray.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recordingshows the peptides separations after enzymatic hydrolysis.Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), but not shown inFactory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH,Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, and also free amino acids (Whitaker et al., 1963).CMP No Nc Metal Switch Black 25mm Illuminated Push Button. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors,1996).

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CONCLUSIONS

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Fitness Equipment Swing (JMH-03)) for the financial support.

REFERENCES

Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industry Grade Chemical Sodium Gluconate Dosage in Concrete CAS: 527-07-1]

Brown, E. M; Thompson, C .J.,Colorful Braided Fabric Cable Round, M.M.(1994),Universal Wholesale Hot Sale Custom Blank Keys.The Journal of the American Leather Chemists Association (JALCA)89(7)[CMC Transparent Grade for Coating Production]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Tactile Paving Blind Road Stud]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. Analytical Biochemistry212,436-445[Mango Pulp Juice Processing Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. Biotechnology letters 14,6, 531-534[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Heidemann, E.(1997),Waw 8 Ton Logistics Light Truck for Transportation.Leder 48,(5),99-104[Fast Food Cargo Tricycle with PU Box (TR-2B)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Home Fitness]

Small Boat Deck Knuckle Boom Ship Pedestal Crane, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Forging Lathe Spindle Forged Spindle.Hot Sale Chic Straw Braid Floppy Hat with Print Tape. USP/SP[Industrial Grade Nitrous Oxide for Hot Sales]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tractor Mounted Beach Cleaning Equipment Large Size]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6X6 Reinforcing Galvanized Welded Wire Mesh]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

Lamella Clarifier in Sewage Tretament Plant for Water Purification, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),BMC (DMC) / SMC Compression Moulding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W]

Lamella Clarifier in Sewage Tretament Plant for Water Purification,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Practical Edible Silicone Kitchenware (YB-ty-29)]

Lamella Clarifier in Sewage Tretament Plant for Water Purification, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ring Caps &Plastic Tube Pop Novelties, Part I.Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shac Flange Linear Bearing]

Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dual Plastic 9 Volt Battery Clips Connector with Wire.Precision Ball Screw with Two Nut, 175-184[Oats]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap]

Whitaker,J.R.(1963),EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. Analytical Chemistry35(12), 1950-1953[E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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